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Low-energy LED red light inhibits the NF-κB pathway and promotes hPDLSCs proliferation and osteogenesis in a TNF-α environment in vitro

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机构: [1]Institute of Stomatology, Southwest Medical University, Luzhou 646000, China [2]The Third Hospital of Mianyang, Department of Stomatology, Mianyang 621000, China [3]Sichuan Mental Health Center, Mianyang 621000, China [4]Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou 646000, China [5]Chenjiaqiao Hospital of Shapingba District Chongqing, Chongqing 400000, China [6]Dazhou Hospital of Integrated TCM & Western Medicine Hospital, Dazhou 635000, China [7]The Third Hospital of Yibin, Department of Stomatology, Yibin 644000, China [8]The Afliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China
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关键词: hPDLSCs  LED red light  TNF-α  Proliferation  Osteogenic diferentiation  NF-κB

摘要:
There are few studies on the effect of low-energy LED red light on periodontal tissue regeneration in an inflammatory environment. In this study, Cell Counting Kit-8 (CCK-8) assays were used to detect the effects of TNF-α at three different concentrations (0, 10 ng/ml, and 20 ng/ml) on the proliferation of human periodontal ligament stem cells (hPDLSCs), and 10 ng/ml was selected as the subsequent experimental stimulation concentration. CCK-8 assays were used to detect the effect of LED red light with energy density of 1 J/ cm2, 3 J/ cm2, and 5 J/cm2 on the proliferation of hPDLSCs. The promotion effect of energy density of 5 J/cm2 on the proliferation of hPDLSCS was the most obvious (p < 0.05). Set CON group, ODM group, ODM + 10 ng/ml TNF-α group, and ODM + 10 ng/ml TNF-α + 5 J/ cm2 LED red light group. Alkaline phosphatase staining and activity detection, alizarin red staining and calcium nodules quantitative detection of osteoblast differentiation products, real-time fluorescence quantitative PCR detection of osteoblast gene expression (Runx2, Col-I, OPN, OCN). The results showed that ODM showed the strongest osteoblast ability, followed by ODM + 10 ng/ml TNF-α + 5 J/ cm2 LED red light group. The osteoblast ability of ODM + 10 ng/ml TNF-α was decreased, but was not found in CON group. Western blot was used to detect the expression of NF-κB pathway protein and osteoblast-related proteins (Runx2, Col-I, OPN, OCN) after addition of PDTC inhibitor. The results showed that the expression of p-IκBα was increased and the expression of IκBα was decreased (p < 0.05). The expression of osteoblast protein increased after the addition of inhibitor (p < 0.05). Therefore, in an inflammatory environment constructed by 10 ng/ml TNF-α, 5 J/cm2 LED red light can upregulate the proliferation and osteogenesis of hPDLSCs by inhibiting NF-κB signaling pathway.© 2023. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.

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出版当年[2023]版:
大类 | 4 区 医学
小类 | 4 区 工程:生物医学 4 区 外科
最新[2023]版:
大类 | 4 区 医学
小类 | 4 区 工程:生物医学 4 区 外科
第一作者:
第一作者机构: [1]Institute of Stomatology, Southwest Medical University, Luzhou 646000, China [2]The Third Hospital of Mianyang, Department of Stomatology, Mianyang 621000, China [3]Sichuan Mental Health Center, Mianyang 621000, China
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通讯机构: [1]Institute of Stomatology, Southwest Medical University, Luzhou 646000, China [4]Oral & Maxillofacial Reconstruction and Regeneration of Luzhou Key Laboratory, Luzhou 646000, China [8]The Afliated Stomatological Hospital of Southwest Medical University, Luzhou 646000, China
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