机构：[1]Department of Urology, Affiliated Hospital of Zunyi Medical University, Zunyi City, Guizhou Province, 563000, China.[2]Department of Pediatric Surgery, Affiliated Hospital of Zunyi Medical University, Zunyi City, Guizhou Province, 563000, China.[3]Department of Urology, Institute of Urology, West China Hospital of Sichuan University, Chengdu City, Sichuan Province, 610000, China.四川大学华西医院[4]Department of Urology, Affiliated Hospital and Clinical Medical College of Chengdu University, Chengdu City, Sichuan Province, 610081, China.
Circular RNAs (circRNAs) contribute to the malignant phenotype and progression of several types of human cancers, including renal cell carcinoma (RCC). This study probed the molecular mechanism of circPGPEP1 regulating RCC proliferation, Warburg effect, and distant metastasis by targeting the miR-378a-3p/JPT1 axis. Here identified higher circPGPEP1 expression in RCC tissues and cells by RT-qPCR, and high levels of circPGPEP1 were positively correlated with high histological grade and distant metastasis in RCC patients. Furthermore, patients with high levels of circPGPEP1 had a worse survival prognosis. Functional assays presented that knockdown of circPGPEP1 inhibited RCC proliferation, invasion, migration, EMT, and Warburg effect. Dual-luciferase reporter assay, RNA immunoprecipitation, nucleoplasmic RNA isolation, and functional rescue experiments confirmed that circPGPEP1 induced JPT1 expression by sponging miR-378a-3p, thereby promoting RCC malignant phenotype. Xenograft assays and metastasis models further demonstrated that down-regulation of circPGPEP1 effectively inhibited tumor growth and distant metastasis of RCC. Taken together, circPGPEP1, a prognostic circRNA in RCC, acts through the miR-378a-3p/JPT1 axis to regulate RCC progression.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2023]版：
大类|4 区生物学
小类|4 区生化与分子生物学
最新[2023]版：
大类|4 区生物学
小类|4 区生化与分子生物学
第一作者：
第一作者机构：[1]Department of Urology, Affiliated Hospital of Zunyi Medical University, Zunyi City, Guizhou Province, 563000, China.
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Wang PeiRui,Chen Jin,Ye Xin,et al.Circ-PGPEP1 augments renal cell carcinoma proliferation, Warburg effect, and distant metastasis[J].Acta biochimica Polonica.2023,70(3):703-711.doi:10.18388/abp.2020_6511.
APA：
Wang PeiRui,Chen Jin,Ye Xin,Wang RuYi,Chen Lin&Zhang HanChao.(2023).Circ-PGPEP1 augments renal cell carcinoma proliferation, Warburg effect, and distant metastasis.Acta biochimica Polonica,70,(3)
MLA：
Wang PeiRui,et al."Circ-PGPEP1 augments renal cell carcinoma proliferation, Warburg effect, and distant metastasis".Acta biochimica Polonica 70..3(2023):703-711
