机构：[1]Department of Radiation Oncology, Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Clinical Research Center for Cancer,Sichuan Cancer Hospital & Institute, Affiliated Cancer Hospital of University of Electronic Science and Technology of China, Chengdu 610000, China四川省人民医院四川省肿瘤医院[2]School of Medicine, University of Electronic Science and Technology of China, Chengdu 610000, China四川省人民医院[3]Department of Oncology, School of Clinical Medicine, Southwest Medical University, Luzhou 646000, China
Abstract: Tumor-associated tertiary lymphoid structures (TLSs) are ectopic lymphoid formations within tumor tissue, with
mainly B and T cell populations forming the organic aggregates. The presence of TLSs in tumors has been strongly associated
with patient responsiveness to immunotherapy regimens and improving tumor prognosis. Researchers have been motivated to
actively explore TLSs due to their bright clinical application prospects. Various studies have attempted to decipher TLSs
regarding their formation mechanism, structural composition, induction generation, predictive markers, and clinical utilization.
Meanwhile, the scientific approaches to qualitative and quantitative descriptions are crucial for TLS studies. In terms of
detection, hematoxylin and eosin (H&E), multiplex immunohistochemistry (mIHC), multiplex immunofluorescence (mIF), and
12-chemokine gene signature have been the top approved methods. However, no standard methods exist for the quantitative
analysis of TLSs, such as absolute TLS count, analysis of TLS constituent cells, structural features, TLS spatial location, density,
and maturity. This study reviews the latest research progress on TLS detection and quantification, proposes new directions for
TLS assessment, and addresses issues for the quantitative application of TLSs in the clinic.
基金：
Key Projects of Sichuan
Natural Science Foundation (No. 2022NSFSC0051), the Clinical
Scientist Program of Sichuan Cancer Hospital (No. YB2022003),
and the Chengdu Technology Innovation R&D Project (No.
2021YF0501659SN), China.
语种：
外文
第一作者：
第一作者机构：[1]Department of Radiation Oncology, Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Clinical Research Center for Cancer,Sichuan Cancer Hospital & Institute, Affiliated Cancer Hospital of University of Electronic Science and Technology of China, Chengdu 610000, China[2]School of Medicine, University of Electronic Science and Technology of China, Chengdu 610000, China
通讯作者：
通讯机构：[1]Department of Radiation Oncology, Radiation Oncology Key Laboratory of Sichuan Province, Sichuan Clinical Research Center for Cancer,Sichuan Cancer Hospital & Institute, Affiliated Cancer Hospital of University of Electronic Science and Technology of China, Chengdu 610000, China[2]School of Medicine, University of Electronic Science and Technology of China, Chengdu 610000, China[3]Department of Oncology, School of Clinical Medicine, Southwest Medical University, Luzhou 646000, China
推荐引用方式(GB/T 7714)：
Man YANG,Yurou CHE,Kezhen LI,et al.肿瘤相关的三级淋巴结构的检测和量化分析[J].Journal of Zhejiang University-Science B（Biomedicine ＆ Biotechnology）.2023,24(09):779-795.
APA：
Man YANG,Yurou CHE,Kezhen LI,Zengyi FANG,Simin LI...&Weidong WANG.(2023).肿瘤相关的三级淋巴结构的检测和量化分析.Journal of Zhejiang University-Science B（Biomedicine ＆ Biotechnology）,24,(09)
MLA：
Man YANG,et al."肿瘤相关的三级淋巴结构的检测和量化分析".Journal of Zhejiang University-Science B（Biomedicine ＆ Biotechnology） 24..09(2023):779-795
