机构：[1]Department of Orthopedics, West China Hospital, West China School of Nursing, Sichuan University, Chengdu 610041, China.四川大学华西医院[2]School of Basic Medical Sciences, Harbin Medical University, Harbin 150081, China.[3]The Ministry of Education Key Laboratory of Clinical Diagnostics, School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.[4]Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, China.四川大学华西医院[5]Laboratory of Dermatology, Clinical Institute of Inflammation and Immunology, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China.四川大学华西医院[6]Key Laboratory of Optoelectronic Technology and Systems, Ministry of Education, Defense Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University, Chongqing 400044, China.[7]State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Macrophages and fibroblasts are two types of important cells in wound healing. The development of novel platforms for studying the interrelationship between these two cells is crucial for the exploration of wound-healing mechanisms and drug development. In this study, a microfluidic chip composed of two layers was designed for the co-culturing of these two cells. An air valve was employed to isolate fibroblasts to simulate the wound-healing microenvironment. The confluence rate of fibroblasts in the co-culture system with different macrophages was explored to reflect the role of different macrophages in wound healing. It was demonstrated that M2-type macrophages could promote the activation and migration of fibroblasts and it can be inferred that they could promote the wound-healing process. The proposed microfluidic co-culture system was designed for non-contact cell-cell interactions, which has potential significance for the study of cell-cell interactions in biological processes such as wound healing, tumor microenvironment, and embryonic development.
基金：
National Natural Science Foundation of China, grant
numbers 81871567 and 62231025.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|3 区工程技术
小类|2 区分析化学2 区仪器仪表3 区纳米科技
最新[2023]版：
大类|3 区工程技术
小类|2 区分析化学3 区仪器仪表3 区纳米科技
第一作者：
第一作者机构：[1]Department of Orthopedics, West China Hospital, West China School of Nursing, Sichuan University, Chengdu 610041, China.
共同第一作者：
通讯作者：
通讯机构：[4]Department of Dermatology, West China Hospital, Sichuan University, Chengdu 610041, China.[5]Laboratory of Dermatology, Clinical Institute of Inflammation and Immunology, Frontiers Science Center for Disease-Related Molecular Network, West China Hospital, Sichuan University, Chengdu 610041, China.
推荐引用方式(GB/T 7714)：
Li Pengcheng,Cui Feiyun,Chen Heying,et al.A Microfluidic Cell Co-Culture Chip for the Monitoring of Interactions between Macrophages and Fibroblasts[J].Biosensors.2022,13(1):doi:10.3390/bios13010070.
APA：
Li Pengcheng,Cui Feiyun,Chen Heying,Yang Yao,Li Gang...&Lyu Xiaoyan.(2022).A Microfluidic Cell Co-Culture Chip for the Monitoring of Interactions between Macrophages and Fibroblasts.Biosensors,13,(1)
MLA：
Li Pengcheng,et al."A Microfluidic Cell Co-Culture Chip for the Monitoring of Interactions between Macrophages and Fibroblasts".Biosensors 13..1(2022)
