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Janus wireframe DNA cube-based 3D nanomachine for rapid and stable fluorescence detection of exosomal microRNA.

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机构： [1]Chongqing Medical University, Chongqing, 400016, China [2]Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China [3]Department of Clinical Laboratory, Chongqing General Hospital, Chongqing, 400016, China [4]Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, China [5]Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016, China [6]Department of Laboratory Medicine, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, 400016, China

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Exosomal microRNAs (miRNAs) have been demonstrated to be credible biomarkers for the diagnosis and monitoring of tumors. Nevertheless, developing simple, rapid, and stable biosensing strategies that are capable of accurately detecting exosomal miRNAs remains a challenge. Herein, an accelerated and biostable three-dimensional (3D) nanomachine based on Janus wireframe DNA cube was constructed for sensitive fluorescence measurement of exosomal miRNA. The Janus wireframe DNA cube could propel target exosomal miRNA-21 rapid movement on its surface by catalytic hairpin assembly (CHA), releasing a massive fluorescence signal. Benefiting from the Janus wireframe DNA cube, the developed 3D nanomachine exhibited significantly improved reaction rate and enhanced biostability in complex biofluids compared to conventional CHA. As a result, this fluorescence biosensing strategy achieved rapid, stable, and single-step detection of exosomal miRNA-21 with the detection limit down to the picomole level. Therefore, this work offers a brief sensing tool for nucleic acid biomarkers detection, which has great application potential in tumor diagnosis.Copyright © 2022 Elsevier B.V. All rights reserved.

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出版当年[2022]版：

大类 | 1 区

工程技术

小类 | 1 区生物工程与应用微生物 1 区分析化学 1 区纳米科技 1 区生物物理 1 区电化学

最新[2023]版：

大类 | 1 区

生物学

小类 | 1 区生物物理 1 区生物工程与应用微生物 1 区分析化学 1 区电化学 2 区纳米科技

第一作者：

第一作者机构： [1]Chongqing Medical University, Chongqing, 400016, China [2]Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China [3]Department of Clinical Laboratory, Chongqing General Hospital, Chongqing, 400016, China [4]Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, China

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通讯作者：

通讯机构： [1]Chongqing Medical University, Chongqing, 400016, China [2]Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China [3]Department of Clinical Laboratory, Chongqing General Hospital, Chongqing, 400016, China [*1]Chongqing Medical University, Chongqing, 400016, China.

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