机构：[1]Laboratory of Anaesthesiology &[2]Critical Care Medicine, Department of Anesthesiology, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.四川大学华西医院[2]Medical College, Tibet University, Lhasa 850000, China.[3]Institute of Clinical Trials, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China.四川大学华西医院[4]Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, Shandong 250012, China.
As a neurotransmitter, norepinephrine (NE) is critical for psychiatric conditions, neurodegenerative diseases, and pheochromocytoma. A real-time and noninvasive method for the detection of NE as a tracer to investigate the NE-relevant disease treatment process is urgently desirable. Herein, we successfully developed a turn-on NE bioluminescent probe (NBP), which was grounded on p-toluenethiol deprotectrf by nucleophilic substitution. Compared with other analytes, the NBP exhibited high sensitivity and selectivity in vitro. More importantly, the NBP provides a promising strategy for in vivo imaging of NE in living animals with noninvasive visualization and real-time features.
基金：
We are grateful for financial support from the National Natural
Science Foundation of China (No. 81773685), the 1.3.5
Project for Disciplines of Excellence, West China Hospital,
Sichuan University (Nos. ZYYC21002 and ZYJC1803), the
Taishan Scholar Program at Shandong Province, the Qilu/
Tang Scholar Program at Shandong University, the Major
Project of Science and Technology of Shandong Province (No.
2015ZDJS04001, China), the Science and Technology Project
of Tibet Autonomous Region (No. XZ201901-GB-08),
Chengdu Science and Technology Program (No. 2018-
CY02-00042-GX), Post-Doctor Research Project, West
China Hospital, Sichuan University (No. 2021HXBH038),
and the Science and Technology Department of Sichuan
Province (2019JDJQ0004 and 2020YFS0004).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2022]版：
大类|1 区化学
小类|1 区分析化学
最新[2023]版：
大类|1 区化学
小类|1 区分析化学
第一作者：
第一作者机构：[1]Laboratory of Anaesthesiology &
通讯作者：
推荐引用方式(GB/T 7714)：
Zuo Zeping,Kang Ting,Hu Shilong,et al.A Bioluminescent Probe for Detecting Norepinephrine in Vivo.[J].Analytical chemistry.2022,94(17):6441-6445.doi:10.1021/acs.analchem.2c00460.
APA：
Zuo Zeping,Kang Ting,Hu Shilong,Su Wuyue,Gan Yu...&Li Minyong.(2022).A Bioluminescent Probe for Detecting Norepinephrine in Vivo..Analytical chemistry,94,(17)
MLA：
Zuo Zeping,et al."A Bioluminescent Probe for Detecting Norepinephrine in Vivo.".Analytical chemistry 94..17(2022):6441-6445
化学