机构：[1]Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000 Sichuan, China [2]Translational Medicine Research Center, North Sichuan Medical College, Nanchong, 637000 Sichuan, China [3]Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, 637000 Sichuan, China [4]Department of Rehabilitation Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000 Sichuan, China [5]University of Electronic Science and Technology of China, Chengdu, 610041 Sichuan, China [6]Department of Clinical Laboratory, Sichuan Cancer Hospital & Institute, Chengdu, 610041 Sichuan, China四川省肿瘤医院
Objective. To investigate the expression of Yin-Yang-1 (YY1) in esophageal carcinoma (ESCA) and its effect on glutamine metabolism in ESCA. Methods. The expression and roles of YY1 in ESCA were investigated using a series of bioinformatics databases and tools. The expression of YY1 between ESCA tissues with the corresponding adjacent tissues was validated using real-time PCR, western blot, and immunohistochemical staining method. Furthermore, the effects of YY1 on ESCC cell proliferation and migration were examined. The correlation between the YY1 and glutamine metabolism was evaluated by western blot. Results. YY1 gene was highly conserved in evolution and upregulated in ESCA tissues and ESCC cell lines (ECA109 and TE-1). In addition, YY1 may affect the level of immune cell infiltration and promote tumor cell immune escape. Functional enrichment analysis found that YY1 involved in many biological processes, such as cell division and glutathione and glutamine metabolism. After siRNA knockdown of YY1 in ECA109 and TE-1, the proliferation and the migration of ECA109 and TE-1 were suppressed. The glutamine consumption and glutamate production were significantly decreased. The protein expression of alanine-, serine-, cysteine-preferring transporter 2 (ASCT2), glutaminase (GLS), and glutamate dehydrogenase (GLUD1) was significantly downregulated. Conclusion. YY1 is highly expressed in ESCA and may promote glutamine metabolism of ESCC cells, indicating it may be as a diagnostic biomarker for ESCA.
基金：
Applied Basic Research Program of Sichuan Province [2021YJ0202]; Medical Research Project of Sichuan [S20048]; Science and Technology Support Program of Nanchong [20YFZJ0113]; key research and development project of the Sichuan Provincial Department of Science and Technology [2022ZYF1927]; Chengdu Municipal Science and Technology Project Science and Technology Bureau [2021-YF05-01792-SN]
第一作者机构：[1]Department of Clinical Laboratory, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000 Sichuan, China [2]Translational Medicine Research Center, North Sichuan Medical College, Nanchong, 637000 Sichuan, China [3]Department of Laboratory Medicine, North Sichuan Medical College, Nanchong, 637000 Sichuan, China
共同第一作者：
通讯作者：
通讯机构：[5]University of Electronic Science and Technology of China, Chengdu, 610041 Sichuan, China [6]Department of Clinical Laboratory, Sichuan Cancer Hospital & Institute, Chengdu, 610041 Sichuan, China
推荐引用方式(GB/T 7714)：
Luo Can,Chen Xin,Bai Yuting,et al.Upregulation of Yin-Yang-1 Associates with Proliferation and Glutamine Metabolism in Esophageal Carcinoma[J].INTERNATIONAL JOURNAL OF GENOMICS.2022,2022:doi:10.1155/2022/9305081.
APA：
Luo, Can,Chen, Xin,Bai, Yuting,Xu, Lei,Wang, Shuqi...&Zhong, Xiaowu.(2022).Upregulation of Yin-Yang-1 Associates with Proliferation and Glutamine Metabolism in Esophageal Carcinoma.INTERNATIONAL JOURNAL OF GENOMICS,2022,
MLA：
Luo, Can,et al."Upregulation of Yin-Yang-1 Associates with Proliferation and Glutamine Metabolism in Esophageal Carcinoma".INTERNATIONAL JOURNAL OF GENOMICS 2022.(2022)
