Prostate cancer is still a public heath priority in men and the impact of this disease will be more pronounced with the aging of the world's population. Clinical heterogeneity of prostate cancer is reflected in spatial and clonal genomic diversity. Accumulating evidence demonstrate that the malignant behavior of cancer is not only attributed to cancer cells, but also fundamentally affected by stromal activity and controlled by various mechanisms of tumor microenvironment. Data of prostate cancer in this study derived from seven GEO datasets and TCGA database. We analyzed the tumor microenvironment of prostate cancer in terms of clinical process, T stage and Gleason score using EPIC and xCell algorithms. We also analyzed the common immune checkpoints. In this study, we confirmed remarkable tumor tissue remodeling in the development of prostate cancer, and further demonstrated the importance of cancer-related fibroblasts in the biochemical recurrence and metastasis for patients with prostate cancer undergoing radical radiotherapy or prostatectomy. In addition, we found that NRP1, CD200, TNFSF18, and CD80 might be the potential targets for prostate cancer.This article is protected by copyright. All rights reserved.