机构：[1]Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.四川大学华西医院[2]Institute of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.四川大学华西医院[3]National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China.四川大学华西医院
Tumor-derived interleukin-8 (IL-8) promotes tumorigenesis and progression of prostate cancer (PCa). MicroRNAs (miRNAs) are noncoding regulatory RNAs and their dysregulation is known to be implicated in carcinogenesis. However, the post-transcriptional mechanism of IL-8 via miRNAs is not fully understood. This study was intended to investigate whether miR-106a could affect the progression of PCa via targeting IL-8 or not.Using bioinformatics analysis, we postulated that IL-8 might be post-transcriptionally regulated by miR-106a. This was validated by dual reporter gene assays that miR-106a could bind to the predicted site of IL-8 mRNA. To determine the biological effects of miR-106a on PCa cells (PC-3 and DU145), MTT, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), migration and invasion assays were performed.We found that miR-106a was barely expressed in PCa cells, whereas IL-8 was aberrantly upregulated. Elevated miR-106a could reduce IL-8 expression by directly binding the 3'-UTR of IL-8. Overexpression of miR-106a in PCa cells triggered cell apoptosis and suppressed cell proliferation, migration, and invasion.This research showed that miR-106a could function as a tumor-suppressor by decreasing IL-8 levels in PCa.2020 Translational Cancer Research. All rights reserved.
基金：
This work was supported by the National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University (Grant number Z2018A01), National Natural Science Foundation of China (Grant number 81672547), and 1.3.5 project for disciplines of excellence, West China Hospital of Sichuan University.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2020]版：
大类|4 区医学
小类|4 区肿瘤学
最新[2023]版：
大类|4 区医学
小类|4 区肿瘤学
第一作者：
第一作者机构：[1]Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.[2]Institute of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.[3]National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu 610041, China.
通讯作者：
通讯机构：[1]Department of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.[2]Institute of Urology, West China Hospital, Sichuan University, Chengdu 610041, China.[*1]Department of Urology and Institute of Urology, West China Hospital, Sichuan University, No. 37 Guoxue Xiang, Chengdu 610041, China.
推荐引用方式(GB/T 7714)：
Shen Pengfei,Sun Guangxi,Zhao Peng,et al.MicroRNA-106a suppresses prostate cancer proliferation, migration and invasion by targeting tumor-derived IL-8.[J].Translational cancer research.2020,9(5):3507-3517.doi:10.21037/tcr.2020.03.70.
APA：
Shen Pengfei,Sun Guangxi,Zhao Peng,Dai Jindong,Zhang Xingming...&Zeng Hao.(2020).MicroRNA-106a suppresses prostate cancer proliferation, migration and invasion by targeting tumor-derived IL-8..Translational cancer research,9,(5)
MLA：
Shen Pengfei,et al."MicroRNA-106a suppresses prostate cancer proliferation, migration and invasion by targeting tumor-derived IL-8.".Translational cancer research 9..5(2020):3507-3517
