机构：[1]School of Mechanical Engineering, Chengdu university, Chengdu 610106, China.[2]Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu 610106, China.[3]Key Laboratory of Coarse Cereal Processing (Ministry of Agriculture and Rural Affairs), School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China.[4]Department of Nephrology, The Third People's Hospital of Chengdu, Chengdu 610031, China.[5]Personalized Drug Therapy Key Laboratory of Sichuan Province, Sichuan Academy of Medical Science and Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610072, China.四川省人民医院
Albumin, the most abundant protein in plasma, has been widely used in drug delivery studies. Here, we developed maleimide-functionalized liposomes (Mal-Lip) that can bind to endogenous albumin to improve the tumor targeting efficiency of liposomes. Transmission electron microscopy and gel electrophoresis studies showed that albumin can bind to Mal-Lip due to the chemical coupling of the albumin thiol groups with the maleimide group. Both conventional liposomes and Mal-Lip showed minimal cytotoxicity within the tested range of lipid concentrations, indicating that the maleimide functionality did not increase the toxicity of liposomes to various cells. Mal-Lip was taken up by 4T1 cells to a greater extent than conventional liposomes, and Mal-Lip accumulated in 4T1 tumors in mice more than conventional liposomes after intravenous injection. These results suggest that the maleimide group can improve the tumor targeting efficiency of liposomes in vivo by binding to endogenous albumin in situ. However, the maleimide group also enhanced the uptake of Mal-Lip by Raw264.7 cells and shortened their time in circulation, indicating that further studies should be performed to prevent elimination of Mal-Lip by the immune system.
第一作者机构：[1]School of Mechanical Engineering, Chengdu university, Chengdu 610106, China.
推荐引用方式(GB/T 7714)：
Li Hanmei,Tang Chuane,Tang Qi,et al.Maleimide-Functionalized Liposomes for Tumor Targeting via In Situ Binding of Endogenous Albumin.[J].JOURNAL OF BIOMEDICAL NANOTECHNOLOGY.2021,17(12):2382-2390.doi:10.1166/jbn.2021.3211.
APA：
Li Hanmei,Tang Chuane,Tang Qi,Yin Dan,He En...&Zhu Yuxuan.(2021).Maleimide-Functionalized Liposomes for Tumor Targeting via In Situ Binding of Endogenous Albumin..JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,17,(12)
MLA：
Li Hanmei,et al."Maleimide-Functionalized Liposomes for Tumor Targeting via In Situ Binding of Endogenous Albumin.".JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 17..12(2021):2382-2390
