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Cryo-EM analysis of Ebola virus nucleocapsid-like assembly.

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机构： [1]The State Key Laboratory of Biotherapy and Cancer Center, Department of Geriatrics and National Clinical Research Centerfor Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610044, China [2]Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA [3]Department of Molecular Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA [4]Department of Bioengineering and Department of Microbiology and Immunology, James H. Clark Center, StanfordUniversity, Stanford, CA 94305, USA [5]Division of Cryo-EM and Bioimaging, SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA 94025, USA [6]Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA

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This protocol describes the reconstitution of the filamentous Ebola virus nucleocapsid-like assembly in vitro. This is followed by solving the cryo-EM structure using helical reconstruction, and flexible fitting of the existing model into the 5.8 Å cryo-EM map. The protocol can be applied to other filamentous viral protein assemblies, particularly those with high flexibility and moderate resolution maps, which present technical challenges to model building. For complete details on the use and execution of this profile, please refer to Su et al. (2018).© 2021 The Author(s).

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第一作者：

第一作者机构： [1]The State Key Laboratory of Biotherapy and Cancer Center, Department of Geriatrics and National Clinical Research Centerfor Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan 610044, China

通讯作者：

通讯机构： [2]Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA [6]Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA

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