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Heart-specific DNA methylation analysis in plasma for the investigation of myocardial damage.

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收录情况： ◇ SCIE

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机构： [1]Biomedical Pioneering Innovation Center, School of Life Sciences, Ministryof Education Key Laboratory of Cell Proliferation and Differentiation,Beijing 100871, China [2]State Key Laboratory of Cardiovascular Disease, FuwaiHospital, National Center for Cardiovascular Diseases, Chinese Academyof Medical Sciences and Peking Union Medical College, Beijing 100871,China [3]Beijing Advanced Innovation Center for Genomics, Peking University,Beijing 100871, China [4]Peking‑Tsinghua Center for Life Sciences, Academyfor Advanced Interdisciplinary Studies, Peking University, Beijing 100871,China [5]National Clinical Research Center for Cardiovascular Diseases, FuwaiHospital, National Center for Cardiovascular Diseases, Chinese Academyof Medical Sciences and Peking Union Medical College, Beijing 100871, China [6]Department of Cardiology, Guangdong Cardiovascular Institute, GuangdongProvincial People’s Hospital, Guangdong Academy of Medical Sciences,Guangzhou, China [7]Department of Cardiology, Qingdao Municipal Hospital,Qingdao, Shandong, China [8]Radiation Oncology Key Laboratory of SichuanProvince, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, Schoolof Medicine, University of Electronic Science and Technology of China,Chengdu, China

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Circulating cell-free DNA (cfDNA) can be released when myocardial damage occurs.Here, we used the methylated CpG tandem amplification and sequencing (MCTA-seq) method for analyzing dynamic changes in heart-derived DNA in plasma samples from myocardial infarction (MI) patients.We identified six CGCGCGG loci showing heart-specific hypermethylation patterns. MCTA-seq deconvolution analysis combining these loci detected heart-released cfDNA in MI patients at hospital admission, and showed that the prominently elevated total cfDNA level after percutaneous coronary intervention (PCI) was derived from both the heart and white blood cells. Furthermore, for the top marker CORO6, we developed a digital droplet PCR (ddPCR) assay that clearly detected heart damage signals in cfDNA of MI patients at hospital admission.Our study provides insights into MI pathologies and developed a new ddPCR assay for detecting myocardial damage in clinical applications.© 2022. The Author(s).

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出版当年[2022]版：

大类 | 2 区医学

小类 | 2 区医学：研究与实验

最新[2023]版：

大类 | 2 区

医学

小类 | 2 区医学：研究与实验

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出版当年[2022]版：

Q1 MEDICINE, RESEARCH & EXPERIMENTAL

最新[2023]版：

Q1 MEDICINE, RESEARCH & EXPERIMENTAL

影响因子： 6.1 最新[2023版] 6.2 最新五年平均 7.4 出版当年[2022版] 6.9 出版当年五年平均 8.448 出版前一年[2021版] 6.1 出版后一年[2023版]

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第一作者机构： [1]Biomedical Pioneering Innovation Center, School of Life Sciences, Ministryof Education Key Laboratory of Cell Proliferation and Differentiation,Beijing 100871, China [3]Beijing Advanced Innovation Center for Genomics, Peking University,Beijing 100871, China [4]Peking‑Tsinghua Center for Life Sciences, Academyfor Advanced Interdisciplinary Studies, Peking University, Beijing 100871,China

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通讯作者：

通讯机构： [1]Biomedical Pioneering Innovation Center, School of Life Sciences, Ministryof Education Key Laboratory of Cell Proliferation and Differentiation,Beijing 100871, China [2]State Key Laboratory of Cardiovascular Disease, FuwaiHospital, National Center for Cardiovascular Diseases, Chinese Academyof Medical Sciences and Peking Union Medical College, Beijing 100871,China [3]Beijing Advanced Innovation Center for Genomics, Peking University,Beijing 100871, China [5]National Clinical Research Center for Cardiovascular Diseases, FuwaiHospital, National Center for Cardiovascular Diseases, Chinese Academyof Medical Sciences and Peking Union Medical College, Beijing 100871, China

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