机构：[1]Institute of Ultrasonic Engineering in Medicine, Chongqing Medical University, Chongqing 400016, China[2]Cancer Center, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610072, China四川省人民医院[3]Department of Oncology, Nanyang First People’s Hospital, Nanyang 473004, China[4]Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX3 9DU, UK
Previous studies have revealed that high-intensity focused ultrasound (HIFU) ablation can trigger an antitumor immune response. The aim of this study was to investigate immune response in tumor-draining lymph nodes (TDLNs) after HIFU treatment. Forty-eight female patients with biopsy-confirmed breast cancer were divided into a control group and an HIFU group. In the control group, 25 patients underwent modified radical mastectomy, but 23 patients in the HIFU group received HIFU ablation of primary cancer, followed by the same operation. Using HE and immunohistochemical staining, the immunologic reactivity pattern and immune cell profile were assessed in paraffin-embedded axillary lymph nodes (ALNs) in all patients. The results showed that ALNs presented more evident immune reactions in the HIFU group than in the control group (100% vs. 64%). Among the ALNs, 78.3% had mixed cellular and humoral immune response, whereas 36% in the control group showed cellular immune response. The numbers of CD3+, CD4+, NK cell, and activated CTLs with Fas ligand+, granzyme+ and perforin+ expression were significantly higher in the ALNs in the HIFU group. It was concluded that HIFU could stimulate potent immune response and significantly increase T cell, activated CTLs and NK cell populations in the TDLNs of breast cancer.
基金：
This research was funded by Ministry of Science and Technology of China, grant number
96-905-02-01.
第一作者机构：[1]Institute of Ultrasonic Engineering in Medicine, Chongqing Medical University, Chongqing 400016, China[2]Cancer Center, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu 610072, China
共同第一作者：
通讯作者：
通讯机构：[1]Institute of Ultrasonic Engineering in Medicine, Chongqing Medical University, Chongqing 400016, China[4]Nuffield Department of Surgical Sciences, University of Oxford, Oxford OX3 9DU, UK
推荐引用方式(GB/T 7714)：
Zhu Xue Qiang,Lu Pei,Xu Zhong Lin,et al.Alterations in Immune Response Profile of Tumor-Draining Lymph Nodes after High-Intensity Focused Ultrasound Ablation of Breast Cancer Patients.[J].CELLS.2021,10(12):doi:10.3390/cells10123346.
APA：
Zhu Xue Qiang,Lu Pei,Xu Zhong Lin,Zhou Qiang,Zhang Jun...&Wu Feng.(2021).Alterations in Immune Response Profile of Tumor-Draining Lymph Nodes after High-Intensity Focused Ultrasound Ablation of Breast Cancer Patients..CELLS,10,(12)
MLA：
Zhu Xue Qiang,et al."Alterations in Immune Response Profile of Tumor-Draining Lymph Nodes after High-Intensity Focused Ultrasound Ablation of Breast Cancer Patients.".CELLS 10..12(2021)
