机构：[1]Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea.[2]Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul, 08826, Republic of Korea.[3]School of Chemical and Biological Engineering, Seoul National University, Seoul, 08826, Republic of Korea.[4]Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ, 08854, USA.[5]Department of Orthopedic Surgery, Korea University Anam Hospital, Seoul, 02841, Republic of Korea.[6]Department of Materials Science and Engineering, Northwestern University, Evanston, IL, 60208, USA.[7]International Institute for Nanotechnology, Evanston, IL, USA.[8]NUANCE Center, Northwestern University, Evanston, IL, USA.[9]KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea.[10]College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials and Engineering, Sichuan University, Chengdu, 610065, China.[11]Department of Chemical and Biological Engineering, Korea University, Seoul, 02841, Republic of Korea.[12]Department of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.[13]Department of Radiology, Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.[14]Department of Bionano Engineering and Bionanotechnology, Hanyang University, Ansan, 15588, Republic of Korea.[15]Department of Biomicrosystem Technology, Korea University, Seoul, 02841, Republic of Korea.
In native microenvironment, diverse physical barriers exist to dynamically modulate stem cell recruitment and differentiation for tissue repair. In this study, we utilize nanoassembly-based magnetic screens of various sizes and elastically tethered them over RGD ligand (cell-adhesive motif)-presenting material surface to generate various nano-gaps between the screens and the RGDs without modulating the RGD density. Large screens exhibiting low RGD distribution stimulate integrin clustering to facilitate focal adhesion, mechanotransduction, and differentiation of stem cells, which were not observed with small screens. Magnetic downward pulling of the large screens decreases nano-gaps, which dynamically suppress the focal adhesion, mechanotransduction, and differentiation of stem cells. Conversely, magnetic upward pulling of the small screens increases the nano-gaps, which dynamically activate focal adhesion, mechanotransduction, and differentiation of stem cells. This regulation mechanism was also shown to be effective in the microenvironment in vivo. Further diversifying geometries of the physical screens could further enable diverse modalities of multifaceted and safe unscreening of the distributed RGDs to unravel and modulate stem cell differentiation for tissue repair. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.
第一作者机构：[1]Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea.
推荐引用方式(GB/T 7714)：
Hong Hyunsik,Min Sunhong,Koo Sagang,et al.Dynamic Ligand Screening by Magnetic Nanoassembly Modulates Stem Cell Differentiation.[J].Advanced materials (Deerfield Beach, Fla.).2021,e2105460.doi:10.1002/adma.202105460.
APA：
Hong Hyunsik,Min Sunhong,Koo Sagang,Lee Yunjung,Yoon Jinho...&Kang Heemin.(2021).Dynamic Ligand Screening by Magnetic Nanoassembly Modulates Stem Cell Differentiation..Advanced materials (Deerfield Beach, Fla.),,
MLA：
Hong Hyunsik,et al."Dynamic Ligand Screening by Magnetic Nanoassembly Modulates Stem Cell Differentiation.".Advanced materials (Deerfield Beach, Fla.) .(2021):e2105460
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