机构：[1]Stem Cell Program, Division of Hematology/Oncology Boston Children’s Hospital BostonUSA[2]Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School Boston USA[3]Precision Medicine Research Center West China Hospital, Sichuan University Chengdu China[4]Department of PediatricsHarvard Medical School BostonUSA[5]Harvard Initiative for RNA Medicine BostonUSA[6]Harvard Stem Cell Institute Cambridge USA
While over 150 distinct types of chemical modifications are known to occur on various cellular RNAs and can be dynamically controlled, the function of most of these modifications remains poorly defined. Collectively, these RNA modifications have been recently termed the "epitranscriptome". Identification and annotation of individual RNA modifications throughout the transcriptome are key for studying the role of the epitranscriptome in the regulation of gene expression and for elucidating the functional relevance of particular RNA modifications in diverse physiological and disease processes. In this protocol, we demonstrate how to identify and annotate RNA modifications based on the informatic analysis of methylated RNA immunoprecipitation and sequencing (MeRIP-seq) data, using RNAmod, a convenient one-stop online interactive platform for the annotation, analysis, and visualization of mRNA modifications.
语种：
外文
PubmedID：
第一作者：
第一作者机构：[1]Stem Cell Program, Division of Hematology/Oncology Boston Children’s Hospital BostonUSA[2]Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School Boston USA
通讯作者：
通讯机构：[1]Stem Cell Program, Division of Hematology/Oncology Boston Children’s Hospital BostonUSA[2]Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School Boston USA
推荐引用方式(GB/T 7714)：
Liu Qi,Lang Xiaoqiang,Gregory Richard I.An Informatics Pipeline for Profiling and Annotating RNA Modifications.[J].Methods in molecular biology (Clifton, N.J.).2021,2298:15-27.doi:10.1007/978-1-0716-1374-0_2.
APA：
Liu Qi,Lang Xiaoqiang&Gregory Richard I.(2021).An Informatics Pipeline for Profiling and Annotating RNA Modifications..Methods in molecular biology (Clifton, N.J.),2298,
MLA：
Liu Qi,et al."An Informatics Pipeline for Profiling and Annotating RNA Modifications.".Methods in molecular biology (Clifton, N.J.) 2298.(2021):15-27
