机构：[1]Lung Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China四川大学华西医院[2]Medical Oncology, West China School of Public Health, Sichuan University,Chengdu, Sichuan 610041, P.R. China[3]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical UniversityGeneral Hospital, Tianjin, China
Accumulating evidence has demonstrated that gene alterations play a crucial role in LUAD development, progression, and prognosis. The current study aimed to identify the hub genes associated with LUAD. In the present study, we used TCGA database to screen the hub genes. Then, we validated the results by GEO datasets. Finally, we used cBioPortal, UALCAN, qRT-PCR, HPA database, TCGA database, and Kaplan-Meier plotter database to estimate the gene mutation, gene transcription, protein expression, clinical features of hub genes in patients with LUAD. A total of 5,930 DEGs were screened out in TCGA database. Enrichment analysis revealed that DEGs were involved in the transcriptional misregulation in cancer, viral carcinogenesis, cAMP signaling pathway, calcium signaling pathway, and ECM-receptor interaction. The combining results of MCODE and CytoHubba showed that ADCY8, ADRB2, CALCA, GCG, GNGT1, and NPSR1 were hub genes. Then, we verified the above results by GSE118370, GSE136043, and GSE140797 datasets. Compared with normal lung tissues, the expression level of ADCY8 and ADRB2 were lower in LUAD tissues, but the expression level of CALCA, GCG, GNGT1, and NPSR1 were higher. In the prognosis analyses, the low expression of ADCY8 and ADRB2 and the high expression of CALCA, GCG, GNGT1, and NPSR1 were correlated with poor OS and poor PFS. The significant differences in the relationship of the expression of 6 hub genes and clinical features were observed. In conclusion, 6 hub genes will not only contribute to elucidating the pathogenesis of LUAD, and may be potential therapeutic targets for LUAD.
Copyright 2021 The Author(s).
基金：
This study was supported by grants from the National Natural Science Foundation of China [grant number 81572288 (to Qinghua
Zhou)]; the Key Project of International Cooperation of Science and Technology Innovation Between Governments, the National
Key Research and Development Plan of China [grant number 2016YEE0103400 (to Qinghua Zhou)].
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|4 区生物学
小类|4 区生化与分子生物学4 区细胞生物学
最新[2023]版：
大类|3 区生物学
小类|3 区生化与分子生物学4 区细胞生物学
第一作者：
第一作者机构：[1]Lung Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
通讯作者：
通讯机构：[1]Lung Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China[3]Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical UniversityGeneral Hospital, Tianjin, China
推荐引用方式(GB/T 7714)：
Zheng Qiangqiang,Min Shihui,Zhou Qinghua.Identification of potential diagnostic and prognostic biomarkers for LUAD based on TCGA and GEO databases.[J].Bioscience reports.2021,41(6):doi:10.1042/BSR20204370.
APA：
Zheng Qiangqiang,Min Shihui&Zhou Qinghua.(2021).Identification of potential diagnostic and prognostic biomarkers for LUAD based on TCGA and GEO databases..Bioscience reports,41,(6)
MLA：
Zheng Qiangqiang,et al."Identification of potential diagnostic and prognostic biomarkers for LUAD based on TCGA and GEO databases.".Bioscience reports 41..6(2021)
