机构：[1]National Engineering Research Center for Biomaterials Sichuan University Chengdu China[2]Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences Shanghai China[3]Center for Molecular Imaging and Translational Medicine, School of Public Health Xiamen University Xiamen China[4]Department of Radiology, West China Hospital Sichuan University Chengdu China四川大学华西医院
Visualizing siRNA delivery through medical imaging methods has drawn much attentions in recent gene therapy studies. Among them, iron oxide-based magnetic resonance imaging (MRI) is regarded as one of the most promising imaging modalities for its high spatial resolution as well as deep penetration and real-time properties. In this chapter, a detailed protocol of an amphiphilic superparamagnetic iron oxide (SPIO) nanovehicle-based siRNA delivery is described, mainly focusing on SPIO/siRNA complexes formation and characterization, in vitro and in vivo siRNA delivery, MRI study of the delivery and transfection efficiency evaluation.
基金：
Creative Research Groups of China
(81621003), National Key Basic Research Program of China
(2013CB933903), National Natural Science Foundation of
China (51903174), Sichuan Science and Technology Program
(2019JDRC0103).
语种：
外文
PubmedID：
第一作者：
第一作者机构：[1]National Engineering Research Center for Biomaterials Sichuan University Chengdu China
通讯作者：
通讯机构：[1]National Engineering Research Center for Biomaterials Sichuan University Chengdu China[4]Department of Radiology, West China Hospital Sichuan University Chengdu China
推荐引用方式(GB/T 7714)：
Jin Rongrong,Zhu Wencheng,Lin Gan,et al.MRI-Visible Nanovehicle for Efficient siRNA Delivery.[J].Methods in molecular biology (Clifton, N.J.).2021,2282:195-208.doi:10.1007/978-1-0716-1298-9_13.
APA：
Jin Rongrong,Zhu Wencheng,Lin Gan,Liu Gang&Ai Hua.(2021).MRI-Visible Nanovehicle for Efficient siRNA Delivery..Methods in molecular biology (Clifton, N.J.),2282,
MLA：
Jin Rongrong,et al."MRI-Visible Nanovehicle for Efficient siRNA Delivery.".Methods in molecular biology (Clifton, N.J.) 2282.(2021):195-208
