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Cytotoxin-Associated Gene A-Positive Helicobacter pylori Promotes Autophagy in Colon Cancer Cells by Inhibiting miR-125b-5p.

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机构： [1]Department of Gastroenterology, e Affiliated Hospital of Southwest Medical University, Luzhou 646000, China [2]Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou 646000, China [3]Department of Gastroenterology, Ya’an People’s Hospital, Ya’an 625000, China [4]Department of Pathology, e Affiliated Hospital of Southwest Medical University, Luzhou 646000, China [5]Department of Cardiovascular Surgery, e Affiliated Hospital of Southwest Medical University, Luzhou 646000, China [6]Cardiovascular and Metabolic Diseases Key Laboratory of Luzhou, Luzhou 646000, China [7]Key Laboratory of Medical Electrophysiology, Ministry of Education & Medical Electrophysiological Key Laboratory of Sichuan Province, (Collaborative Innovation Center for Prevention of Cardiovascular Diseases) Institute of Cardiovascular Research, Southwest Medical University, Luzhou 646000, China

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To investigate the effects of cytotoxin-associated gene A- (CagA-) positive Helicobacter pylori on proliferation, invasion, autophagy, and expression of miR-125b-5p in colon cancer cells. Colon cancer cells were cocultured with H. pylori (CagA+) to analyze the effects of H. pylori on miR-125b-5p and autophagy. Colon cancer cells infected with H. pylori (CagA+) were mimicked by transfection of CagA plasmid. The effects of CagA on the proliferation, invasion, and autophagy of colon cancer cells were analyzed. Cell counting kit-8 (CCK-8), clone formation, and Transwell assays were used to detect cell viability, proliferation, and invasion ability, respectively. Proteins and miRNAs were detected by western blotting and qPCR, respectively. H. pylori (CagA+) inhibited expression of miR-125b-5p and promoted autophagy in colon cancer cells. MiR-125 b-5p was underexpressed in colon cancer cells after CagA overexpression. CagA promoted colon cancer cell proliferation, invasion, and autophagy. Overexpression of miR-125b-5p inhibited the proliferation, invasion, and autophagy of colon cancer cells and reversed the effects of CagA. H. pylori (CagA+) infection may promote the development and invasion of colon cancer by inhibiting miR-125b-5p. Copyright © 2021 Xiaolin Zhong et al.

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出版当年[2021]版：

大类 | 4 区医学

小类 | 4 区传染病学 4 区微生物学

最新[2023]版：

大类 | 4 区医学

小类 | 4 区传染病学 4 区微生物学

第一作者：

第一作者机构： [1]Department of Gastroenterology, e Affiliated Hospital of Southwest Medical University, Luzhou 646000, China [2]Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou 646000, China

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通讯作者：

通讯机构： [5]Department of Cardiovascular Surgery, e Affiliated Hospital of Southwest Medical University, Luzhou 646000, China [6]Cardiovascular and Metabolic Diseases Key Laboratory of Luzhou, Luzhou 646000, China [7]Key Laboratory of Medical Electrophysiology, Ministry of Education & Medical Electrophysiological Key Laboratory of Sichuan Province, (Collaborative Innovation Center for Prevention of Cardiovascular Diseases) Institute of Cardiovascular Research, Southwest Medical University, Luzhou 646000, China

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