Essential oil from the raw and vinegar-processed Rhizoma Curcumae ameliorate CCl4-induced liver fibrosis: integrating network pharmacology and molecular mechanism evaluation.
机构：[1]State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan, China[2]Institute of Thai-Chinese Medicine Department of Thai Traditional and Alternative Medicines, Ministry of Public Health, Bangkok 11000, Thailand[3]Huachiew TCM Hospital, Bangkok 10100, Thailand
Liver fibrosis, caused by multiple chronic liver injuries, is a known contributor to cirrhosis and even liver cancer. As a Traditional Chinese Medicine (TCM), Rhizoma curcumae has been extensively used in the treatment of liver fibrosis with satisfying therapeutic effects; however, its mechanism is unclear. The essential oil is the main bioactive component. The purpose of this study was to investigate the chemical profile and the pharmacological mechanisms of the essential oil of Rhizoma curcumae (EORC) against liver fibrosis by combining network pharmacology and transcriptomic technologies. A total of 37 active compounds were identified using the GC/MS system and literature mining, and the corresponding putative targets were predicted. Then, network pharmacology method was applied to identify the 168 candidate targets of EORC-alleviated liver fibrosis. String database and Cytoscape software were used to build the herb-compound-target network and protein-protein interactions (PPIs) network. Functional and pathway enrichment analysis indicated that EORC significantly influenced TGF-β1/Smads and PI3K/AKT pathways. Experimentally, we verified that EORC attenuated the severity and pathological changes during liver fibrosis progression based on the CCl4-induced liver fibrosis rat model. Transcriptomic technologies demonstrated that EORC ameliorated liver fibrosis partially by regulating the TGF-β1/Smads and PI3K/AKT pathways. In addition, the effect of vinegar-processed EORC was more significant than that of the raw one. Therefore, EORC can alleviate the severity of liver fibrosis through mechanisms predicted by network pharmacology and provide a basis for the further understanding of the application of EORC in the treatment of liver fibrosis.
基金：
This work was supported by the National Natural Science
Foundation of China (Grant No. 81403103, 82073994); the
International Cooperation Project of Science and Technology
Department of Sichuan Province (Grant No. 2018HH0122);
Chengdu Science and Technology Bureau International Science
and Technology Cooperation Project (Grant No. 2017-GH02-
00054-HZ); the Outstanding Young Scientific and Technological
Talents Project of Science and Technology Department of
Sichuan Province (Grant No. 2020JDJQ0049); the Key Open
Fund of Key Laboratory of Coarse Grain Processing, Ministry of
Agriculture (Grant No. 2019CC02); the Xinglin Scholar Research
Premotion Project of Chengdu University of TCM (Grant No.
XSGG2019005, QNXZ2018026); Science and Technology
Transformation Project of Chengdu University of Traditional
Chinese Medicine (Grant No. CGPY1605).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|2 区医学
小类|2 区生化与分子生物学2 区食品科技
最新[2023]版：
大类|1 区农林科学
小类|2 区生化与分子生物学2 区食品科技
第一作者：
第一作者机构：[1]State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Chen Yi,Liao Wan,Zhu Zongping,et al.Essential oil from the raw and vinegar-processed Rhizoma Curcumae ameliorate CCl4-induced liver fibrosis: integrating network pharmacology and molecular mechanism evaluation.[J].Food & function.2021,12(9):4199-4220.doi:10.1039/d0fo03052j.
APA：
Chen Yi,Liao Wan,Zhu Zongping,Chen Jiao,Yang Qingsong...&Li Rui.(2021).Essential oil from the raw and vinegar-processed Rhizoma Curcumae ameliorate CCl4-induced liver fibrosis: integrating network pharmacology and molecular mechanism evaluation..Food & function,12,(9)
MLA：
Chen Yi,et al."Essential oil from the raw and vinegar-processed Rhizoma Curcumae ameliorate CCl4-induced liver fibrosis: integrating network pharmacology and molecular mechanism evaluation.".Food & function 12..9(2021):4199-4220
农林科学