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Exosomes derived from colon cancer cells and plasma of colon cancer patients promote migration of SW480 cells through Akt/mTOR pathway.

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机构： [1]School of Medicine, Southwest Jiaotong University, The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu 610031, Sichuan, China. [2]Medical Research Center, The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, The Second Chengdu Hospital Affiliated to Chongqing Medical University, Chengdu 610031, Sichuan, China. [3]Department of Digestive Diseases, The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, The Second Chengdu Hospital Affiliated to Chongqing Medical University, Chengdu 610031, Sichuan, China. [4]Department of Gastroenterology and Hepatology, Sichuan Cancer Hospital and Institute, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610000, Sichuan, China. [5]Chengdu First People’ s Hospital, Chengdu, 610041, Sichuan, China

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To investigate the effect of exosomes derived from colon cancer (CC) cells and plasma of CC patients on migration of SW480 cells. The exosomes derived from culture medium of human colon epithelial cell line NCM460 and CC cell line SW620 were isolated by ultracentrifugation. The exosomes derived from plasma of CC patients and healthy controls were isolated by size exclusion chromatography (SEC). The particle size and morphology of exosomes were identified by nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) respectively, and exosomal markers were detected by Western blotting. The uptake of fluorescent DiI labeled exosomes by SW480 cells was observed by confocal microscopy. Transwell assay was used to detect the effect of exosomes on the migration of SW480 cells. The expression level of associated proteins in signaling pathway were analyzed by Western blotting. Rapamycin, an inhibitor of mTOR, was used to study the role of mTOR signaling pathway on exosomes mediated migration of SW480 cells. The results of NTA and TEM showed that the particle size of the isolated exosomes was about 120 nm, which were small vesicles with membrane structure. The expressions of exosomal markers Alix, TSG101 and CD63 could be detected. The exosomes were evidenced by a red fluorescent signal inside the cytoplasm of SW480 recipient cells, and could promote the migration of SW480 cells, which is associated with Akt/mTOR signaling pathway. Compared with the control group, plasma exosomes derived from CC patients could significantly promote the migration of SW480 cells. Inhibition the activity of mTOR signaling could attenuate the migration of SW480 cells. Exosomes derived from CC cells and plasma of CC patients could promote the migration of SW480 cells, which is associated with Akt/mTOR signaling pathway. Copyright © 2021. Published by Elsevier GmbH.

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出版当年[2021]版：

大类 | 4 区医学

小类 | 4 区病理学

最新[2023]版：

大类 | 4 区医学

小类 | 4 区病理学

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出版当年[2021]版：

Q2 PATHOLOGY

最新[2023]版：

Q2 PATHOLOGY

影响因子： 2.9 最新[2023版] 2.6 最新五年平均 3.309 出版当年[2021版] 2.864 出版当年五年平均 3.25 出版前一年[2020版] 2.8 出版后一年[2022版]

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第一作者机构： [1]School of Medicine, Southwest Jiaotong University, The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu 610031, Sichuan, China.

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通讯作者：

通讯机构： [2]Medical Research Center, The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, The Second Chengdu Hospital Affiliated to Chongqing Medical University, Chengdu 610031, Sichuan, China. [5]Chengdu First People’ s Hospital, Chengdu, 610041, Sichuan, China [*1]Medical Research Center, The Affiliated Hospital of Southwest Jiaotong University, The Third People’s Hospital of Chengdu, The Second Chengdu Hospital Affiliated to Chongqing Medical University, 82 Qinglong Street, Chengdu 610031, Sichuan, China. [*2]Department of Digestive Diseases, The First People’s Hospital of Chengdu, School of Medicine, Southwest Jiaotong University, 18 Wanxiang North Road, Gaoxin District, Chengdu 610041, Sichuan, China

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