机构：[1]Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, The Third People’s Hospital of Chengdu, Chengdu, Sichuan, China[2]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau(SAR), China[3]Department of Cardiothoracic Surgery, The Affiliated Hospital, Southwest Medical University, No. 25, Taiping St., Luzhou, Sichuan, China[4]Department of Pulmonary and Critical Care Medicine, Chengdu Third People’s Hospital Branch of National Clinical Research Centre for Respiratory Disease, Affiliated Hospital of Chongqing Medical University, Chengdu, Sichuan, China[5]Department of Pulmonary and Critical Care Medicine, Hospital of Sichuan Friendships, Chengdu, Sichuan, China
Golgi membrane protein 1 (GOLM1) was implicated in carcinogenesis of multiple types of cancer. However, Phosphoproteome landscapes of GOLM1 overexpression in lung cancer remain largely unknown. In this study, using data from the Cancer Genome Atlas (TCGA) and phosphoproteome, we systematically evaluated the feature of GOLM1 and studied its prognostic value in non-small cell lung cancer (NSCLC). The proliferation, migration, and invasion capacities in PC9 cell with GOLM1 overexpression were determined using Trans-well system assay. Tumor engrafts was visualized in mice models and confirmed by ex vivo. An increased expression of GOLM1 had shorter overall survival (OS) in patients with NSCLC in TCGA database. GOLM1 in single gene set enrichment analysis (GSEA) related to adherent's junction, cell cycle, and pathway in cancer. Overexpression of GOLM1 in GOLM1OE PC9 cells promoted cell proliferation, migration, and invasion. Decreased migration and invasion potential were also observed in knockdown of GOLM1 in GOLM1KD PC9 cells in migration assay. An increased expression of GOLM1 could significantly increase the growth of tumor in xenograft mice models. phosphoproteome analysis showed 239 upregulated and 331 downregulated Phosphorylated proteins in GOLM1OE PC9 cells. Overexpression of GOLM1 in GSEA was significantly related to P53 in MAPK signaling pathway. Overexpression of GOLM1enhanced the phosphorylation of P53 protein at site S315 but inhibited the formation of P53 tetramers. These results indicate that overexpression GOLM1 enhances non-small-cell carcinoma aggressiveness through inhibited the formation of P53 tetramer.
基金：
This work is supported by the Macao Science and Technology Development
Fund (Project code: 0096/2018/A3), National Natural Science Foundation of
China (81970026 and 82000029), project of Chengdu high level clinical key
specialty, Luzhou government and Southwest Medical University Cooperative
Project (No. 2016lzxnyd-z01), Science and Technology Department of Sichuan Province (2018JY0380), and Health Commission of Sichuan Province
(19ZD002), Health Commission of Chengdu (2019077 and 2019078).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2021]版：
大类|2 区医学
小类|3 区细胞生物学
最新[2023]版：
大类|2 区生物学
小类|2 区细胞生物学
第一作者：
第一作者机构：[1]Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, The Third People’s Hospital of Chengdu, Chengdu, Sichuan, China[2]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau(SAR), China[3]Department of Cardiothoracic Surgery, The Affiliated Hospital, Southwest Medical University, No. 25, Taiping St., Luzhou, Sichuan, China
共同第一作者：
通讯作者：
通讯机构：[1]Laboratory of Allergy and Precision Medicine, Chengdu Institute of Respiratory Health, The Third People’s Hospital of Chengdu, Chengdu, Sichuan, China[2]State Key Laboratory of Quality Research in Chinese Medicine, Macau University of Science and Technology, Taipa, Macau(SAR), China[4]Department of Pulmonary and Critical Care Medicine, Chengdu Third People’s Hospital Branch of National Clinical Research Centre for Respiratory Disease, Affiliated Hospital of Chongqing Medical University, Chengdu, Sichuan, China
推荐引用方式(GB/T 7714)：
Song Qi,He Xiang,Xiong Ying,et al.The functional landscape of Golgi membrane protein 1 (GOLM1) phosphoproteome reveal GOLM1 regulating P53 that promotes malignancy.[J].Cell death discovery.2021,7(1):42.doi:10.1038/s41420-021-00422-2.
APA：
Song Qi,He Xiang,Xiong Ying,Wang Junlan,Zhang Lei...&Li Guoping.(2021).The functional landscape of Golgi membrane protein 1 (GOLM1) phosphoproteome reveal GOLM1 regulating P53 that promotes malignancy..Cell death discovery,7,(1)
MLA：
Song Qi,et al."The functional landscape of Golgi membrane protein 1 (GOLM1) phosphoproteome reveal GOLM1 regulating P53 that promotes malignancy.".Cell death discovery 7..1(2021):42
