This study was designed to investigate the effects of peroxisome proliferator-activated receptor delta (PPAR delta) on the proliferation and apoptosis of human colorectal cancer cells.
For RNA interfering (RNAi), HCT-116 cells were transfected with short hairpin RNA (shRNA)-expressing plasmids against PPAR delta or negative control vectors, and the stably transfected cells were selected with G418. The efficacy of RNAi was assessed by real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blotting analysis. The proliferation, cell cycle, and apoptosis of HCT-116 cells treated by RNAi, compared with those containing control vectors or untreated, were analyzed respectively by using MTT (methyl thiazolyl tetrazolium), flow cytometry, and TdT (terminal deoxynucleotidyl transferase)-mediated dUTP nick end-labeling (TUNEL) assay.
RNAi targeting PPAR delta resulted in substantial suppression of PPAR delta expression and significantly promoted the proliferation of HCT-116 cells relative to those with control vectors or untreated, obviously decreasing the frequency of G1-phase cells but had no effect on cell apoptosis.
PPAR delta may inhibit the proliferation of CRC cells and increase the number of cells in G1 phase, without any function in cell apoptosis.
基金：
China NSFC Research Fund (30400434); China
Medical Board of New York (CMB) Fund (96636).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2008]版：
大类|3 区医学
小类|2 区外科3 区胃肠肝病学
最新[2023]版：
大类|2 区医学
小类|2 区外科3 区胃肠肝病学
第一作者：
第一作者机构：[1]Institute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Sichuan Province, China
通讯作者：
通讯机构：[1]Institute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Sichuan Province, China[*1]Institute of Digestive Surgery and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 1 Keyuan Road 4, Gaopengdadao, Chengdu 610041, Sichuan Province, China.
推荐引用方式(GB/T 7714)：
Yang L,Zhou Z G,Zheng X L,et al.RNA interference against peroxisome proliferator-activated receptor delta gene promotes proliferation of human colorectal cancer cells.[J].Diseases of the colon and rectum.2008,51(3):318-26; discussion 326-8.doi:10.1007/s10350-007-9145-8.
APA：
Yang L,Zhou Z G,Zheng X L,Wang L,Yu Y Y...&Li Y.(2008).RNA interference against peroxisome proliferator-activated receptor delta gene promotes proliferation of human colorectal cancer cells..Diseases of the colon and rectum,51,(3)
MLA：
Yang L,et al."RNA interference against peroxisome proliferator-activated receptor delta gene promotes proliferation of human colorectal cancer cells.".Diseases of the colon and rectum 51..3(2008):318-26; discussion 326-8
