机构：[1]Institute for Nanobiomedical Technology and Membrane Biology, State Key Lab of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University, No 1, Keyuan 4 Lu, Gaopeng Avenue, Hi-Tech Zone, Chengdu 610041, China四川大学华西医院[2]School of Light Industry and Food, Sichuan University, Chengdu 610065, China[3]Mathematical College, Sichuan University, Chengdu 610064, China
A new method applying sensitive and selective liquid chromatography coupled with mass spectrometry (LC/MS/MS) for analyzing tertiary-butylhydroquinone (TBHQ) and its metabolites in rat serum was validated. Using an extracted ion chromatogram (EIC) of m/z 149, free TBHQ was observed in rat serum after dosing TBHQ at 350 mg/kg to male and female Sprague-Dawley (SD) rats. Four major metabolites of TBHQ were identified-a TBHQ-sulfate, two TBHQ-sulfate-derived substances and a TBHQ-glucuronide through MS(n) spectra. Besides its simplicity, the sample treatment allows one to obtain a very good recovery of analysts, namely around 95%. This result suggests that the method described here is useful for the analysis of TBHQ and its metabolites in rat serum. Moreover, the metabolism of TBHQ was investigated using the method. After oral administration, TBHQ appear to be more completely absorbed and bio-transformed by males than females, which may result in higher acute oral toxicity of TBHQ for males than females.
基金：
Financial support of this research was from the
National Key Technologies R&D Program of China with grant no.
2001BA804A21. Sincere thanks are to Dr Pengpenhao at Peking
University for providing technical assistance in the experimental
studies.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2008]版：
大类|4 区生物
小类|4 区生化与分子生物学4 区营养学
最新[2023]版：
大类|4 区医学
小类|4 区生化与分子生物学4 区营养学
第一作者：
第一作者机构：[1]Institute for Nanobiomedical Technology and Membrane Biology, State Key Lab of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University, No 1, Keyuan 4 Lu, Gaopeng Avenue, Hi-Tech Zone, Chengdu 610041, China
通讯作者：
推荐引用方式(GB/T 7714)：
Huang Wen,Gu Yinchun,Niu Hai.Determination of tertiary-butylhydroquinone and its metabolites in rat serum by liquid chromatography-ion trap mass spectrometry.[J].Lipids.2008,43(3):281-8.doi:10.1007/s11745-007-3135-4.
APA：
Huang Wen,Gu Yinchun&Niu Hai.(2008).Determination of tertiary-butylhydroquinone and its metabolites in rat serum by liquid chromatography-ion trap mass spectrometry..Lipids,43,(3)
MLA：
Huang Wen,et al."Determination of tertiary-butylhydroquinone and its metabolites in rat serum by liquid chromatography-ion trap mass spectrometry.".Lipids 43..3(2008):281-8
