机构：[1]Department of Pathology, Institute of Pathology, West China Hospital, Sichuan University, Chengdu, China四川大学华西医院[2]Genetic Laboratory, West China Second University Hospital, Sichuan University, Chengdu, China[3]State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China四川大学华西医院
A novel nude mice model of human extranodal nasal type NK/T-cell lymphoma was established by subcutaneously implanting the sample taken from the patient with secondary extranodal nasal type NK/T-cell lymphoma of the stomach into the right axillary region of a BALB/c (nu/nu) nude mouse. This model had been successfully transplanted in vivo for thirty-two generations with a stable growth cycle. The survival rates of both resuscitation and transplantation were 100%. Histologically, the tumor cells were medium to large size and arranged in sheets, with a little mesenchyma, and disseminated almost in all passages of the lymphoma-bearing nude mice. Immunologically, the tumor cells were positive for CD56, cytoplasmic CD3, granzyme B or TIA-1 and LMP1, sometimes for CD8 but negative for surface CD3, CD7, CD20 and CD1a. EBER1/2 was found. No T-cell receptor gamma gene rearrangement was detected in the transplanted tumors. Furthermore, both human sequencing-tagged sites SY14 and Y chromosome were detected by PCR or fluorescent in situ hybridization, respectively, in the transplanted tumor. The transplanted tumor in this novel nude mice model maintained the essential features of human extranodal nasal type NK/T-cell lymphoma, and it would be an ideal tool in vivo for further research of the tumor.
基金：
National Natural Science Foundation of China
(30470747 and 30570769).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2008]版：
大类|1 区医学
小类|2 区血液学2 区肿瘤学
最新[2023]版：
大类|1 区医学
小类|1 区血液学1 区肿瘤学
第一作者：
第一作者机构：[1]Department of Pathology, Institute of Pathology, West China Hospital, Sichuan University, Chengdu, China
通讯作者：
通讯机构：[1]Department of Pathology, Institute of Pathology, West China Hospital, Sichuan University, Chengdu, China[*1]Department of Pathology, Institute of Pathology, West China Hospital, Sichuan University, No. 37 Guoxuexiang, Chengdu, Sichuan 610041, China
推荐引用方式(GB/T 7714)：
Zhao S,Tang Q L,He M X,et al.A novel nude mice model of human extranodal nasal type NK/T-cell lymphoma.[J].Leukemia.2008,22(1):170-8.doi:10.1038/sj.leu.2404945.
APA：
Zhao S,Tang Q L,He M X,Yang F,Wang H...&Liu W P.(2008).A novel nude mice model of human extranodal nasal type NK/T-cell lymphoma..Leukemia,22,(1)
MLA：
Zhao S,et al."A novel nude mice model of human extranodal nasal type NK/T-cell lymphoma.".Leukemia 22..1(2008):170-8
医学