机构：[1]Institute for Nanobiomedical Technology and Membrane Biology, State Key Lab of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China四川大学华西医院[2]Department of Environmental Engineering, Peking University, Beijing 100871, China[3]Mathematical College, Sichuan University, Chendu 610064, China[4]China Agricultural University, Beijing 100083, China
Conversion of acorn fringe extract into ellagic acid production by Aspergillus oryzae and Endomyces fibuliger were investigated. The results showed that ellagic acid production was maximized when co-fermentation of the two fungi was performed at 30 degrees C and pH 5.0 with 5.7 g/l of initial substrate concentration, which were close to the optimal values for both fungi to yield an appropriate consortium of hydrolytic enzymes. Meanwhile, it was found that the co-fermentation could compensate the deficiencies in the level of polyphenol oxidase activity from pure A. oryzae and the levels of ellagitannin acyl hydrolase and beta-glucosidase activities from pure E. fibuliger, resulting in. 0.91 g/l of biomass concentration containing 1.84 g/l of ellagic acid. The research not only demonstrates that the co-fermentation is an effective approach to utilize forest byproduct for ellagic acid production, but also provides more evidences for understanding evolution of ellagic acid production with enzymes actions, which is important for process control of ellagic acid production in industrial application.
基金：
Financial support of this research was from the National
Natural Science Foundation of China with Grant
No. 40371011. Sincere thanks are to Dr. Jinshui Yang at
China Agricultural University for providing the microbial
culture and technical assistance in the experimental studies
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2008]版：
大类|2 区工程技术
小类|1 区农业工程2 区能源与燃料3 区生物工程与应用微生物
最新[2023]版：
大类|1 区环境科学与生态学
小类|1 区农业工程1 区生物工程与应用微生物1 区能源与燃料
第一作者：
第一作者机构：[1]Institute for Nanobiomedical Technology and Membrane Biology, State Key Lab of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China
通讯作者：
推荐引用方式(GB/T 7714)：
Huang Wen,Li Zhenshan,Niu Hai,et al.Utilization of acorn fringe for ellagic acid production by Aspergillus oryzae and Endomyces fibuliger.[J].Bioresource technology.2008,99(9):3552-8.doi:10.1016/j.biortech.2007.07.047.
APA：
Huang Wen,Li Zhenshan,Niu Hai,Li Lulu,Lin Wensheng&Yang Jinshui.(2008).Utilization of acorn fringe for ellagic acid production by Aspergillus oryzae and Endomyces fibuliger..Bioresource technology,99,(9)
MLA：
Huang Wen,et al."Utilization of acorn fringe for ellagic acid production by Aspergillus oryzae and Endomyces fibuliger.".Bioresource technology 99..9(2008):3552-8
环境科学与生态学