机构：[1]Laboratory of Biomedical Ultrasonics, Institute of Women and Children’s Health, West China Second University Hospital, Sichuan University, Chengdu[2]Institute of Ultrasound Engineering in Medicine[3]Department of Rehabilitation - 1st Affiliated Hospital, Chongqing Medical University, Chongqing, China重庆医科大学附属第一医院
An increased capacity for DNA repair plays a very important role in cisplatin (DDP) resistance in ovarian cancers. Ultrasound is a potential chemotherapy sensitizer. The aim of this study was to determine whether ultrasound in conjunction with chemotherapy increases DNA damage in chemoresistant human ovarian cancer cells.
Ultrasound and/or cyclosporin A were used to overcome chemoresistance in a DDP-resistant human ovarian cancer cell line, COC1/DDP. DNA damage was quantified by comet assay, a form of single-cell gel electrophoresis in which the length of the comet tail reflects the level of DNA damage.
Neither ultrasound nor cyclosporin A alone led to detectable DNA breakage. The use of ultrasound increased DNA breakage due to DDP, while the use of cyclosporin A did not. The addition of ultrasound and cyclosporin A in conjunction with DDP resulted in a 2.55 times increase in the length of comet tail compared with using DDP alone, while their combined use resulted in a 1.73 times increase compared with the combination of just DDP and insonation.
Insonation increases DNA breakage attributable to DDP in chemoresistant human ovarian cancer cells, and might sensitize cyclosporin A.
(c) 2009 ISUOG. Published by John Wiley & Sons, Ltd.
基金：
Natural
Science Foundation of China (30670550 and 10774198)
and the Ministry of Education (NCET-05-0775 and
SRFDP 20060631001).
语种：
外文
被引次数：
WOS：
PubmedID：
中科院(CAS)分区：
出版当年[2009]版：
大类|3 区医学
小类|1 区声学2 区妇产科学3 区核医学
最新[2023]版：
大类|1 区医学
小类|1 区声学1 区妇产科学1 区核医学
JCR分区：
出版当年[2009]版：
Q1ACOUSTICSQ1OBSTETRICS & GYNECOLOGYQ1RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
最新[2023]版：
Q1ACOUSTICSQ1OBSTETRICS & GYNECOLOGYQ1RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
第一作者机构：[1]Laboratory of Biomedical Ultrasonics, Institute of Women and Children’s Health, West China Second University Hospital, Sichuan University, Chengdu[2]Institute of Ultrasound Engineering in Medicine[*1]Institute of Ultrasound Engineering in Medicine, Chongqing Medical University, Chongqing 400016, China
共同第一作者：
通讯作者：
通讯机构：[1]Laboratory of Biomedical Ultrasonics, Institute of Women and Children’s Health, West China Second University Hospital, Sichuan University, Chengdu[2]Institute of Ultrasound Engineering in Medicine[*1]Institute of Ultrasound Engineering in Medicine, Chongqing Medical University, Chongqing 400016, China
推荐引用方式(GB/T 7714)：
Yu T,Yang Y,Liu S,et al.Ultrasound increases DNA damage attributable to cisplatin in cisplatin-resistant human ovarian cancer cells.[J].ULTRASOUND IN OBSTETRICS & GYNECOLOGY.2009,33(3):355-359.doi:10.1002/uog.6258.
APA：
Yu T,Yang Y,Liu S&Yu H.(2009).Ultrasound increases DNA damage attributable to cisplatin in cisplatin-resistant human ovarian cancer cells..ULTRASOUND IN OBSTETRICS & GYNECOLOGY,33,(3)
MLA：
Yu T,et al."Ultrasound increases DNA damage attributable to cisplatin in cisplatin-resistant human ovarian cancer cells.".ULTRASOUND IN OBSTETRICS & GYNECOLOGY 33..3(2009):355-359
医学