机构：[1]Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, P.R. China, 610041.四川大学华西医院[2]Department of Thoracic Surgery, West China Hospital, Sichuan University, P.R. China, 610041.四川大学华西医院[3]Tianjin Lung Cancer Institute,Tianjin Medical University General Hospital, Tianjin 300052, China
To investigate the selective killing efficacy of the double suicide genes driven by KDR promoter.
A double suicide gene system with the KDR promoter, pcDNA3-KDRp-CDglyTK, was constructed and transfected into lung cancer cell lines L9981 and NL9980, and human hepatocellular carcinoma cell line HepG2. The efficiency and specificity of the double suicide gene system were assayed by in vitro cellular proliferation and apoptosis, as well as in vivo xenograft studies.
The transgenic CD and TK genes were only expressed in L9981 and NL9980 but not in HepG2 cells. Pre-treating transfected cells with 5-Fc and GCV significantly reduced proliferation, enhanced apoptosis in L9981 and NL9980 but not in HepG2 cells. The tumor formed by L9981 and NL9980 cells with the double suicide gene system was much smaller in vivo.
Tumor targeted expression of CDglyTK gene driven by KDR promotor represents a novel strategy for effective gene therapy of tumor with intrinsic KDR.
基金：
The authors would like to thank Dr. JinFeng DING for kindly providing the
CD and TK plasmid; this work was supported by the National Natural
Science Foundation of China (No.30872547, to Guowei Che).
语种：
外文
PubmedID：
第一作者：
第一作者机构：[1]Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, P.R. China, 610041.
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推荐引用方式(GB/T 7714)：
Ma Junrong,Li Mi,Mei Longyong,et al.Double suicide genes driven by kinase domain insert containing receptor promoter selectively kill human lung cancer cells.[J].Genetic vaccines and therapy.2011,9:6.doi:10.1186/1479-0556-9-6.
APA：
Ma Junrong,Li Mi,Mei Longyong,Zhou Qinghua,Liu Lunxu...&Che Guowei.(2011).Double suicide genes driven by kinase domain insert containing receptor promoter selectively kill human lung cancer cells..Genetic vaccines and therapy,9,
MLA：
Ma Junrong,et al."Double suicide genes driven by kinase domain insert containing receptor promoter selectively kill human lung cancer cells.".Genetic vaccines and therapy 9.(2011):6
