机构：[1]Laboratory of Genetics, West China Institute of Maternal and Child Health, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China.[2]Laboratory of Cell and Gene Therapy, West China Institute of Maternal and Child Health, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China.[3]Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China.[4]Prenatal Diagnosis Center of Sichuan Province, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China.[5]Key Laboratory of Obstetrics, Gynecology, Pediatric Diseases and Birth Defects of Ministry of Education, Chengdu 610041, P. R. China.[6]Division of Medical Genetics, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
Background: Intraperitoneal (i.p.) models that accurately mimic the feature behavior of human ovarian cancer are
required to investigate the pathology and therapeutics of the disease. However, established i.p. models which are
well-characterized and reliable are few. The purposes of this study are to establish a representative mice i.p. model
of the disease and to analyze the consequent pathology.
Methods: Fresh tumor cells fiom the ascites of patient were injected into female NOD/SCID mice intraperitoneally.
Histology, Cytogenetic, immunohistochemistry,tumor markers of CA125,AFP, CA-199 and CEA were used to analyze
the model.
Results: The mice developed marked abdominal distention within 6 months after inoculated with tumor cells from a
patient with epithelial ovarian carcinoma. The mice developed clinically evident intraperitoneal tumors and massive
ascites containing numerous tumor cells in clumps. CA125 level in our model was high in both serum and ascites
supernatants, while levels of other tumor markers, such as AFP, CA-199 and CEA, were normal. Cytogenetic analysis and
immunohistochemical staining confirmed its characteristics resembling human epithelial ovarian tumor.
Conclusions: The model described in this paper accurately mimics the features of ovarian tumor, which may be useful
for evaluation of new therapeutics.
Intraperitoneal (i.p.) models that accurately mimic the feature behavior of human ovarian cancer are required to investigate the pathology and therapeutics of the disease. However, established i.p. models which are well-characterized and reliable are few. The purposes of this study are to establish a representative mice i.p. model of the disease and to analyze the consequent pathology.
Fresh tumor cells fiom the ascites of patient were injected into female NOD/SCID mice intraperitoneally. Histology, Cytogenetic, immunohistochemistry,tumor markers of CA125,AFP, CA-199 and CEA were used to analyze the model.
The mice developed marked abdominal distention within 6 months after inoculated with tumor cells from a patient with epithelial ovarian carcinoma. The mice developed clinically evident intraperitoneal tumors and massive ascites containing numerous tumor cells in clumps. CA125 level in our model was high in both serum and ascites supernatants, while levels of other tumor markers, such as AFP, CA-199 and CEA, were normal. Cytogenetic analysis and immunohistochemical staining confirmed its characteristics resembling human epithelial ovarian tumor.
The model described in this paper accurately mimics the features of ovarian tumor, which may be useful for evaluation of new therapeutics.
基金：
National Natural Science Foundation of China
grants (30973461, 81270660, 30772323) awarded to HW and SL L, the
Science Grant from Science and Technology Department of Sichuan
Province, China (2008SZ0018, 2012SZ0136) awarded to SL L. This work was
also partly supported by Beijing Yi Ke Wan Yun Technology Co. Ltd.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2013]版：
大类|3 区生物
小类|3 区生殖生物学
最新[2023]版：
大类|3 区医学
小类|3 区生殖生物学
第一作者：
第一作者机构：[1]Laboratory of Genetics, West China Institute of Maternal and Child Health, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China.[3]Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China.[5]Key Laboratory of Obstetrics, Gynecology, Pediatric Diseases and Birth Defects of Ministry of Education, Chengdu 610041, P. R. China.
通讯作者：
通讯机构：[1]Laboratory of Genetics, West China Institute of Maternal and Child Health, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China.[2]Laboratory of Cell and Gene Therapy, West China Institute of Maternal and Child Health, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China.[3]Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China.[4]Prenatal Diagnosis Center of Sichuan Province, West China Second University Hospital, Sichuan University, Chengdu 610041, P. R. China.[5]Key Laboratory of Obstetrics, Gynecology, Pediatric Diseases and Birth Defects of Ministry of Education, Chengdu 610041, P. R. China.
推荐引用方式(GB/T 7714)：
Jianjun Zhang,Xinlian Chen,Gang Shi,et al.Establishment of a new representative model of human ovarian cancer in mice.[J].Journal of ovarian research.2013,6(1):9.doi:10.1186/1757-2215-6-9.
APA：
Jianjun Zhang,Xinlian Chen,Gang Shi,Xiaoyan Xie,Hongqian Liu...&He Wang.(2013).Establishment of a new representative model of human ovarian cancer in mice..Journal of ovarian research,6,(1)
MLA：
Jianjun Zhang,et al."Establishment of a new representative model of human ovarian cancer in mice.".Journal of ovarian research 6..1(2013):9
