A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array.
机构:[1]Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University,[2]State Key Laboratory of Optical Technologies for Microfabrication, Institute of Optics and Electronics, Chinese Academy of Science, Chengdu, Sichuan, People’s Republic of China
Squamous cell carcinoma antigen (SCCa), as a tumor biomarker, plays an important role in adjuvant diagnosis, treatment evaluation, and prognosis prediction for cervical cancer patients. Localized surface plasmon resonance (LSPR) technique based on noble metal nanoparticles bypasses the disadvantages of traditional testing strategies, in terms of free-labeling, short assay time, good sensitivity, and selectivity. Herein, we develop a novel and reusable LSPR biosensor for the detection of SCCa. First, a triangle-shaped silver nanoparticle array was fabricated using the nanosphere lithography method. Next, we investigated and verified the feasibility of amino coupling method using 11-mercaptoundecanoic acid (MUA) to form a functionalized chip surface with monoclonal anti-SCCa antibodies on the silver nanoparticles for distinct detection of SCCa. Different concentrations of SCCa were successfully tested in both buffer and human serum by the ultrasensitive and specific LSPR system, with a linear quantitative detection range of 0.1-1,000 pM under optimal conditions. With appropriate regeneration solution, for example 50 mM glycine-HCl (pH 2.0), the LSPR biosensor featured effective fabrication reproducibility, which reduced both production cost and testing time. Our study represents the first application of the LSPR biosensor in cervical cancer, and demonstrates that the rapid, simple, and reusable nanochip can serve as a potential alternative for clinical serological diagnosis of SCCa in cervical cancer patients.
基金:
This project was financially supported by National Key
Basic Research Program of China (2011CB301800) and
Sichuan Provincial Science and Technology Support Project
(2012SZ0021).
语种:
外文
PubmedID:
中科院(CAS)分区:
出版当年[2014]版:
大类|2 区工程技术
小类|2 区药学3 区纳米科技
最新[2023]版:
大类|2 区医学
小类|2 区药学3 区纳米科技
第一作者:
第一作者机构:[1]Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University,
通讯作者:
通讯机构:[1]Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University,[*1]Department of Gynecology and Obstetrics, West China Second University Hospital, Sichuan University, Chengdu, China. No 20, Block 3, Renminnan Road, Chengdu, Sichuan, People’s Republic of China (610041)
推荐引用方式(GB/T 7714):
Qianying Zhao,Ruiqi Duan,Jialing Yuan,et al.A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array.[J].International journal of nanomedicine.2014,9:1097-104.doi:10.2147/IJN.S58499.
APA:
Qianying Zhao,Ruiqi Duan,Jialing Yuan,Yi Quan,Huan Yang&Mingrong Xi.(2014).A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array..International journal of nanomedicine,9,
MLA:
Qianying Zhao,et al."A reusable localized surface plasmon resonance biosensor for quantitative detection of serum squamous cell carcinoma antigen in cervical cancer patients based on silver nanoparticles array.".International journal of nanomedicine 9.(2014):1097-104