机构：[1]Laboratory of Molecular Translational Medicine, West China Institute of Women and Children’s Health, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan, China[2]Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan, China[3]Department of Anesthesiology, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan, China
Preeclampsia is associated with inadequate invasion of trophocytes and spiral artery remodeling. As a β-D-glucuronidase enzyme, Heparanase is related to tumor angiogenesis, development and invasion. Trophocytes have similar characteristics to tumor cells, and heparanase could therefore play an important role in the pathogenesis of preeclampsia.
The expression of heparanase in severe preeclampsia and normal placentas was detected via real-time PCR, immunohistochemistry and western blotting. The effects of heparanase on trophocytes migration and invasion were investigated by culturing the HTR-8/Svneo cell line with recombinant human heparanase protein in vitro.
The levels of inactive 65-kDa heterologous heparanase dimers were obviously increased, and the content of the 50-kDa active polypeptide was decreased in severe preeclampsia. Furthermore, exogenous heparanase protein could reduce the migration and invasion of HTR-8/Svneo cells.
Our results suggested that heparanase might be an important factor in the pathogenesis of severe preeclampsia.
基金：
the grants from the Sichuan Provincial Science & Technology Project (No. 2014JY0081), the Office of Science & Technology of Chengdu (No. 2014-HM01-00043-SF) and the Research Fund for the Doctoral Program of Higher Education of China (No. 20120181120046).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|4 区医学：研究与实验
最新[2023]版：
无
第一作者：
第一作者机构：[1]Laboratory of Molecular Translational Medicine, West China Institute of Women and Children’s Health, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan, China
通讯作者：
通讯机构：[3]Department of Anesthesiology, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan, China[*1]Department of Anesthesiology, West China Second University Hospital, Sichuan University, 20#, 3rd Segment, Ren Min Nan Lu, Chengdu 610041, Sichuan, P. R. China
推荐引用方式(GB/T 7714)：
Wang Yan-Yun,Zhou Rong,Zhou Bin,et al.Overexpression of heparanase is associated with preeclampsia by inhibiting invasion of trophocytes.[J].International journal of clinical and experimental medicine.2015,8(10):18107-14.
APA：
Wang Yan-Yun,Zhou Rong,Zhou Bin,Wang Tao,Zhang Lin&Luo Dong.(2015).Overexpression of heparanase is associated with preeclampsia by inhibiting invasion of trophocytes..International journal of clinical and experimental medicine,8,(10)
MLA：
Wang Yan-Yun,et al."Overexpression of heparanase is associated with preeclampsia by inhibiting invasion of trophocytes.".International journal of clinical and experimental medicine 8..10(2015):18107-14
