机构：[1]Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Linjiang Road 76, Chongqing 400010, People’s Republic of China[2]Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong 637000, Sichuan, People’s Republic of China.
Dysregulation of microRNA-150 (miR-150) is commonly observed in solid tumor and has been reported to be involved in multiple important biological processes, such as cell proliferation, apoptosis, and metastasis. Elevated miR-150 level was also detected in cervical carcinoma, whereas its function in cancer progression has not been studied yet.
The expression of miRNA-150 in cervical carcinoma was compared with normal cervical tissue and using qRT-PCR. The effects of miR-150 on cell cycle and apoptosis, as well as the expression of cycle- and apoptosis-related genes, were determined using flow cytometry, TUNEL assay, qRT-PCR, and Western blot, respectively. The direct target of miR-150 was confirmed using 3' untranslated region (UTR) luciferase reporter assay.
miR-150 promotes cervical cancer cell survival and growth, while the inhibition of miR-150 suppresses these actions. miR-150 also induced the cell cycle progression from G1/G0 to S phase, resulting in an enhancement of growth. Several cell cycle- and apoptosis-related genes, CyclinD1, p27, BIM, and FASL were modulated by miR-150. Moreover, miR-150 directly reduced the expression of FOXO4, which regulates the expression of CyclinD1, p27, BIM, and FASL, by targeting its 3' UTR.
Taken together, our data demonstrated that elevated miR-150 targets FOXO4 expression and therefore regulates multiple genes expression, resulting in cervical cancer cell growth and survival.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|3 区生物
小类|4 区生化与分子生物学
最新[2023]版：
无
第一作者：
第一作者机构：[1]Department of Obstetrics and Gynecology, The Second Affiliated Hospital, Chongqing Medical University, Linjiang Road 76, Chongqing 400010, People’s Republic of China[2]Department of Obstetrics and Gynecology, The Second Clinical Medical Institute of North Sichuan Medical College, Nanchong 637000, Sichuan, People’s Republic of China.
通讯作者：
推荐引用方式(GB/T 7714)：
Li Jun,Hu Lina,Tian Chao,et al.microRNA-150 promotes cervical cancer cell growth and survival by targeting FOXO4.[J].BMC molecular biology.2015,16:24.doi:10.1186/s12867-015-0052-6.
APA：
Li Jun,Hu Lina,Tian Chao,Lu Feng,Wu Jia&Liu Li.(2015).microRNA-150 promotes cervical cancer cell growth and survival by targeting FOXO4..BMC molecular biology,16,
MLA：
Li Jun,et al."microRNA-150 promotes cervical cancer cell growth and survival by targeting FOXO4.".BMC molecular biology 16.(2015):24
