机构：[1]Laboratory of Molecular Translational Medicine, West China Institute ofWomen and Children’s Health, Key Laboratory of Obstetric & Gynecologicand Pediatric Diseases and Birth Defects of Ministry of Education, West ChinaSecond University Hospital, Sichuan University, Chengdu, Sichuan, P R China[2]Department of Urology, West China Hospital, Sichuan University, Chengdu,Sichuan, P R China四川大学华西医院[3]Department of Urology, Affiliated Hospital of NorthSichuan Medical College, Nanchong, Sichuan, P R China[4]Department ofUrology, Institute of oncology, the Second People’s Hospital of Sichuan,Chengdu, P R China[5]Department of Forensic Biology, West China School ofPreclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, P RChina
Interleukin-27 (IL-27) has been recognized as a pleiotropic cytokine with both pro- and anti-inflammatory properties. Few studies have investigated polymorphisms and serum/plasma levels of IL-27 in diseases including cancers. This study has analyzed the associations of IL-27 gene polymorphisms, as well as plasma levels of IL-27, with susceptibility to bladder cancer and clinical outcome.
Three hundred and thirty-two patients (nonmuscle-invasive bladder cancer (NMIBC)/muscle-invasive bladder cancer (MIBC): 176/156) included in a 60-month follow-up program and 499 controls were enrolled. Two single nucleotide polymorphisms (SNPs), rs153109 and rs17855750, were genotyped by polymerase chain reaction (PCR) -restriction fragment length polymorphism (RFLP) method. Plasma concentration of IL-27 was determined by ELISA in 124 patients (NMIBC/MIBC: 50/74) and 151 controls.
Significantly increased risk for bladder cancer was associated with AG/GG genotypes of rs153109 (P = 0.029). No GG genotype of rs17855750 was observed in controls, while 4 patients were found to be GG homozygotes, suggesting GG genotype may be associated with bladder cancer risk (P = 0.006). For bladder cancer patients, SNP rs17855750 was also associated with increased risk for MIBC. For MIBC patients, but not NMIBC, TG/GG genotypes of rs17855750 turned out to be a protective factor for overall survival (P = 0.035). Significantly reduced plasma levels of IL-27 were observed in both NMIBC and MIBC patients compared with controls (P < 0.0001).
Our data suggest that polymorphisms and reduced plasma levels of IL-27 may predict the susceptibility to bladder cancer, and rs17855750 may be a useful marker to distinguish patients with high risk of death.
基金：
This work was supported by the National Natural Science Foundation of
China (No. 81172440, No. 81172494, No. 81272821 and No. 81202023); the
Applied Basic Research Programs of Science and Technology Commission
Foundation of Sichuan Province (No. 2012SZ0008); and the Science
Foundation for The Excellent Youth Scholars of Sichuan University
(No. 2011SCU04A16).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|3 区医学
小类|3 区肿瘤学
最新[2023]版：
大类|2 区医学
小类|3 区肿瘤学
第一作者：
第一作者机构：[1]Laboratory of Molecular Translational Medicine, West China Institute ofWomen and Children’s Health, Key Laboratory of Obstetric & Gynecologicand Pediatric Diseases and Birth Defects of Ministry of Education, West ChinaSecond University Hospital, Sichuan University, Chengdu, Sichuan, P R China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Zhou Bin,Zhang Peng,Tang Tielong,et al.Polymorphisms and plasma levels of IL-27: impact on genetic susceptibility and clinical outcome of bladder cancer.[J].BMC cancer.2015,15:433.doi:10.1186/s12885-015-1459-7.
APA：
Zhou Bin,Zhang Peng,Tang Tielong,Liao Hong,Zhang Kui...&Zhang Lin.(2015).Polymorphisms and plasma levels of IL-27: impact on genetic susceptibility and clinical outcome of bladder cancer..BMC cancer,15,
MLA：
Zhou Bin,et al."Polymorphisms and plasma levels of IL-27: impact on genetic susceptibility and clinical outcome of bladder cancer.".BMC cancer 15.(2015):433
医学