Tenascin-C expression is associated with poor prognosis in hepatocellular carcinoma (HCC) patients and the inflammatory cytokine TNF-α-induced TNC expression promotes migration in HCC cells.
机构：[1]Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu 610041, China四川大学华西医院[2]Division ofInfectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, China[3]MolecularBiology and Lung Cancer Program, Lovelace Respiratory Research Institute, 2425 Ridgecrest Dr. SE, Albuquerque,NM 87108, USA[4]Biorepository, State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, China
出处：
ISSN：
摘要：
Although tenascin-c (TNC) in inflammatory microenvironment contributes to progression in some tumors, its role in hepatocellular carcinoma (HCC) in metastasis and the mechanism by which TNC expression is regulated in HCC cells are elusive. In this study, we examined TNC expression in 100 HCC tissue samples by immunohistochemistry and compared which between the groups with or without metastasis. TNC expression was higher in metastatic HCC tissues than that in the non-metastatic HCC tissues, which was associated with the Knodell inflammation scores. Importantly, high level of TNC expression was associated with lower survival rate and shorter survival time in the HCC patients. We then investigated the mechanism by which TNC expression is regulated in HCC cells with an in vitro cell culture system. The recombinant TNF-α and conditioned medium from macrophages induced TNC expression at both mRNA and protein levels in HepG2 cells. The induction of TNC expression by conditioned medium from macrophages was suppressed by a TNF-α neutralizing antibody. TNF-α-promoted cell migration was inhibited by a TNC siRNA. In addition, TNF-α-induced TNC expression was blocked by a NF-κB pathway inhibitor. These results suggest that TNF-α in the tumor microenvironment induces TNC expression in HCC cells through the NF-κB pathway, which in turn, promotes HCC cell migration. Thus, TNC may play an important role in promoting HCC metastasis and TNC expression could be a predictive factor for poor prognosis in HCC patients.
基金：
This study was
supported by the research Grants from the
National Natural Sciences Foundation of China
(No. 81101602).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|2 区医学
小类|3 区肿瘤学
最新[2023]版：
大类|3 区医学
小类|3 区肿瘤学
第一作者：
第一作者机构：[1]Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu 610041, China[2]Division ofInfectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, China
通讯作者：
通讯机构：[1]Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu 610041, China[2]Division ofInfectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, Chengdu 610041, China[*1]Center of Infectious Diseases, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Wuhou District, Chengdu, 610041, Sichuan, China
推荐引用方式(GB/T 7714)：
Nong Yunhong,Wu Dongbo,Lin Yong,et al.Tenascin-C expression is associated with poor prognosis in hepatocellular carcinoma (HCC) patients and the inflammatory cytokine TNF-α-induced TNC expression promotes migration in HCC cells.[J].American journal of cancer research.2015,5(2):782-91.
APA：
Nong Yunhong,Wu Dongbo,Lin Yong,Zhang Yongqiang,Bai Lang&Tang Hong.(2015).Tenascin-C expression is associated with poor prognosis in hepatocellular carcinoma (HCC) patients and the inflammatory cytokine TNF-α-induced TNC expression promotes migration in HCC cells..American journal of cancer research,5,(2)
MLA：
Nong Yunhong,et al."Tenascin-C expression is associated with poor prognosis in hepatocellular carcinoma (HCC) patients and the inflammatory cytokine TNF-α-induced TNC expression promotes migration in HCC cells.".American journal of cancer research 5..2(2015):782-91
