机构：[1]Department of Radiology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.[2]Department of Radiology, People's Liberation Army 520 Hospital, Mianyang, Sichuan 621000, P.R. China.[3]Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Jinan, Shandong 250117, P.R. China.
The present study was conducted to investigate the 11C‑Choline metabolic mechanism and examine the association between 11C‑Choline metabolism and uptake in different pathological types of lung cancer. A total of 18 tumor specimens and corresponding normal lung tissues were collected from patients who were diagnosed with lung cancer using 11C‑Choline positron emission tomography (PET)/computed tomography (CT) imaging between January 2007 and December 2008 at the Medical Imaging Center of the Provincial Hospital Affiliated to Shandong University. The diagnosis was further confirmed pathologically following surgery. Reverse transcription polymerase chain reaction and western blotting were used to investigate the expression of choline acetyltransferase (ChAT) and choline kinase α (ChoK) in lung cancer tissue and normal lung tissue. The 11C‑Choline PET/CT data were analyzed visually and semiquantitatively. Compared with the expression in the normal lung tissues, the mRNA and protein expression of ChAT and ChoK increased in nine and 14 of the 18 lung tumors, respectively. A total of eight of the 18 tumors exhibited significantly increased expression, while three exhibited no expression of ChoK and ChAT. All lung cancer lesions were visualized with 11C‑Choline PET/CT imaging. The phosphorylation and acetylation pathways of choline metabolism may be important in 11C‑Choline uptake and metabolism in different pathological types of lung cancer.
基金：
National Natural Science Foundation of China (grant nos. 81001223 and 81301868), the Shandong Province Science and Technology Development Plan (grant nos. 2012GSF11820, 2012YD18053 and 2012YD18086) and the Medical Science and Technology Project of Shandong Province (grant no. 2009QW014).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2015]版：
大类|4 区医学
小类|4 区医学：研究与实验4 区肿瘤学
最新[2023]版：
大类|3 区医学
小类|4 区医学：研究与实验4 区肿瘤学
第一作者：
第一作者机构：[1]Department of Radiology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.
通讯作者：
通讯机构：[1]Department of Radiology, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China.[*1]Department of Radiology, Provincial Hospital Affiliated to Shandong University, 324 Jingwu Road, Jinan, Shandong 250021, P.R. China
推荐引用方式(GB/T 7714)：
Huang Zhaoqin,Rui Jun,Li Xin,et al.Use of ¹¹C-Choline positron emission tomography/computed tomography to investigate the mechanism of choline metabolism in lung cancer.[J].Molecular medicine reports.2015,11(5):3285-90.doi:10.3892/mmr.2015.3200.
APA：
Huang Zhaoqin,Rui Jun,Li Xin,Meng Xiangjiao&Liu Qingwei.(2015).Use of ¹¹C-Choline positron emission tomography/computed tomography to investigate the mechanism of choline metabolism in lung cancer..Molecular medicine reports,11,(5)
MLA：
Huang Zhaoqin,et al."Use of ¹¹C-Choline positron emission tomography/computed tomography to investigate the mechanism of choline metabolism in lung cancer.".Molecular medicine reports 11..5(2015):3285-90
