详情页 - 四川省肿瘤医院知识库

当前位置：首页 > 详情页

TAGLN2, a novel regulator involved in Hepatitis B virus transcription and replication.

文献详情

资源类型：

Pubmed体系：

作者：

机构： [a]Department of Forensic Pathology, West China School of Basic Science and Forensic Medicine, Sichuan University, 17 3rd Renmin Road, Chengdu, 610041, China [b]Division of Infectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, 37 Guoxuexiang, Chengdu, 610041, China [c]Center of Infectious Diseases, West China Hospital, Sichuan University, 37 Guoxuexiang, Chengdu, 610041, China [d]Comprehensive Pneumology Center, Institute of Lung Biology and Disease, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany

出处：

DOI：

ISSN：

摘要：

Hepatitis B virus (HBV) infection is one of the major health problems in the world. Transgelin-2 (TAGLN2) expression has been revealed to be significantly altered in previous studies concerning HBV-host interaction. The present study investigated TAGLN2 expression patterns in HBV related hepatocellular carcinoma (HCC) tissues and its role in HBV transcription and replication. We collected 59 HBV related HCC tissue samples, their adjacent non-tumoral tissues and 16 normal livers to make the tissue microarray. TAGLN2 protein was detected by immunohistochemistry and the transcriptional levels of TAGLN2, HBc, HBs and HBx were detected by qRT-PCR. Then we investigated the function of TAGLN2 on HBV transcription and replication in vitro by ectopic expressing or knocking down TAGLN2 in HepG2 and HepG2.2.15 cell lines. We further studied the effect of HBx on TAGLN2 expression with a Tet-on HBx expressing cell line. TAGLN2 protein expression was lower in normal livers and HBV-HCC tissues comparing to adjacent non-tumoral tissues. The transcriptional levels of TAGLN2 in HBV-HCC tissues and their adjacent tissues were positively related to that of HBc, HBs and HBx (P < 0.05). Ectopic expression of TAGLN2 in vitro could enhance HBV transcription and replication while suppressing TAGLN2 had the contrary effect. TAGLN2 could be induced by HBx in a dose-dependent manner. Our data demonstrated that TAGLN2 might be an HBx induced positive host factor involved in HBV transcription and replication and HBx related liver fibrosis and tumorigenesis. Copyright © 2016 Elsevier Inc. All rights reserved.

基金：

语种：

PubmedID：

中科院(CAS)分区：

出版当年[2016]版：

大类 | 3 区生物

小类 | 4 区生化与分子生物学 4 区生物物理

最新[2023]版：

大类 | 3 区生物学

小类 | 3 区生物物理 4 区生化与分子生物学

第一作者：

第一作者机构： [a]Department of Forensic Pathology, West China School of Basic Science and Forensic Medicine, Sichuan University, 17 3rd Renmin Road, Chengdu, 610041, China

共同第一作者：

通讯作者：

通讯机构： [b]Division of Infectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, 37 Guoxuexiang, Chengdu, 610041, China [*1]Division of Infectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, 37 Guoxuexiang, Chengdu, 610041, China

推荐引用方式(GB/T 7714)：

APA：

MLA：