Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression.
机构：[1]Department of Anesthesia and Critical Care Medicine Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.四川大学华西医院[2]Institute of Neuroscience and Experiment Animal Center, Kunming Medical University, Kunming 650031, China.[3]Center for Experimental Technology for Preclinical Medicine, Chengdu Medical College, Chengdu 610083, Sichuan, China.
Bone marrow mesenchymal stem cell (BMSCs)-based therapy seems to be a promising treatment for acute lung injury, but the therapeutic effects of BMSCs transplantation on acute lung injury induced by brain ischemia and the mechanisms have not been totally elucidated. This study explores the effects of transplantation of BMSCs on acute lung injury induced by focal cerebral ischemia and investigates the underlying mechanism.
Acute lung injury model was induced by middle cerebral artery occlusion (MCAO). BMSCs (with concentration of 1 × 10(6)/ml) were transplanted into host through tail vein 1 day after MCAO. Then, the survival, proliferation and migration of BMSCs in lung were observed at 4 days after transplantation, and histology observation and lung function were assessed for 7 days. Meanwhile, in situ hybridization (ISH), qRT-PCR and western blotting were employed to detect the expression of TNF-α in lung.
Neurobehavioral deficits and acute lung injury could be seen in brain ischemia rats. Implanted BMSCs could survive in the lung, and relieve pulmonary edema, improve lung function, as well as down regulate TNF-α expression.
The grafted BMSCs can survive and migrate widespread in lung and ameliorate lung injury induced by focal cerebral ischemia in the MCAO rat models. The underlying molecular mechanism, at least partially, is related to the suppression of TNF-α.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2016]版：
大类|4 区医学
小类|4 区行为科学4 区神经科学
最新[2023]版：
大类|2 区心理学
小类|1 区行为科学2 区神经科学
第一作者：
第一作者机构：[1]Department of Anesthesia and Critical Care Medicine Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Anesthesia and Critical Care Medicine Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, Sichuan, China.[2]Institute of Neuroscience and Experiment Animal Center, Kunming Medical University, Kunming 650031, China.
推荐引用方式(GB/T 7714)：
Qin‑qin He,Xiang He,Yan‑ping Wang,et al.Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression.[J].Behavioral and brain functions : BBF.2016,12(1):9.doi:10.1186/s12993-016-0093-0.
APA：
Qin‑qin He,Xiang He,Yan‑ping Wang,Yu Zou,Qing‑jie Xia...&Ting‑hua Wang.(2016).Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression..Behavioral and brain functions : BBF,12,(1)
MLA：
Qin‑qin He,et al."Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression.".Behavioral and brain functions : BBF 12..1(2016):9
