Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57.
机构：[1]School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China[2]Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen, China[3]Stanley Ho Center for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China[4]Shenzhen Center for Disease Control and Prevention (Shenzhen CDC), Shenzhen, China深圳医学信息中心中国医学科学院肿瘤医院深圳医院[5]Institute of Infectious Diseases, The 3rd Peoples’ Hospital of Shenzhen, Shenzhen, China[6]State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University , Chengdu, China四川大学华西医院[7]School of Biomedical Sciences, The University of Hong Kong, Hong Kong, China[8]College of Letter and Sciences, University of California at Berkeley, Berkeley, CA, USA[9]Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China
There is no effective drug to treat EV71 infection yet. Traditional Chinese herbs are great resources for novel antiviral compounds. Here we showed that Oblongifolin M (OM), an active compound isolated from Garcinia oblongifolia, potently inhibited EV71 infection in a dose dependent manner. To identify its potential effectors in the host cells, we successfully identified 18 proteins from 52 differentially expressed spots by comparative proteomics studies. Further studies showed that knockdown of ERp57 inhibited viral replication through downregulating viral IRES (internal ribosome entry site) activities, whereas ectopic expression of ERp57 increased IRES activity and partly rescued the inhibitory effects of OM on viral replication. We demonstrated that OM is an effective antiviral agent; and that ERp57 is one of its cellular effectors against EV71 infection.
基金：
The work was partly supported by The National
Basic Research Development Program (Grant No.
2013CB911302), Grants from National Science
Foundation of China (81471964), Grants from The
Science Technology and Innovation Committee of
Shenzhen Municipality (JCYJ20130401172046448,
JCYJ20120615085419420, JSGG20151030110921727),
and the general research grant of Hong Kong (RGC-GRF
Nos. 14105214, 464512, 11100215) to Dr. Ming-Liang HE;
and by grant of The Signifi cant New Drug Development
from National Major Scientifi c and Technological Special
Project (No. 2013ZX09103002-020) to Dr. Hong-xi Xu
第一作者机构：[1]School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China
共同第一作者：
通讯作者：
推荐引用方式(GB/T 7714)：
Mengjie Wang,Qi Dong,Hua Wang,et al.Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57.[J].ONCOTARGET.2016,7(8):8797-8808.doi:10.18632/oncotarget.7122.
APA：
Mengjie Wang,Qi Dong,Hua Wang,Yaqing He,Ying Chen...&Ming-Liang He.(2016).Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57..ONCOTARGET,7,(8)
MLA：
Mengjie Wang,et al."Oblongifolin M, an active compound isolated from a Chinese medical herb Garcinia oblongifolia, potently inhibits enterovirus 71 reproduction through downregulation of ERp57.".ONCOTARGET 7..8(2016):8797-8808
医学