机构：[1]Department of Neurosurgery, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation center, Chengdu, 610041, PR China.四川大学华西医院[2]Department of Pharmacology, Yale School of Medicine, Yale University, New Haven, Connecticut, 06520, USA.[3]Institute of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, PR China.四川大学华西医院
Combination chemotherapy is an important protocol in glioma therapy and honokiol shows synergistic anticancer effects with doxorubicin. In this paper, honokiol (HK) and doxorubicin (Dox) co-loaded Methoxy poly(ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL) nanoparticles were prepared with a assembly method. The particle size (about 34 nm), morphology, X-ray Powder Diffraction (XRD), in vitro release profile, cytotoxicity and cell proliferation effects were studied in detail. The results indicated that honokiol and doxorubicin could be efficiently loaded into MPEG-PCL nanoparticles simultaneously, and could be released from the micelles in an extended period in vitro. In addition, honokiol and doxorubicin loaded in MPEG-PCL nanoparticles could efficiently suppress glioma cell proliferation and induce cell apoptosis in vitro. Furthermore, Dox-HK-MPEG-PCL micelles inhibited glioma growth more significantly than Dox-MPEG-PCL and HK-MPEG-PCL in both nude mice and zebrafish tumor models. Immunohistochemical analysis indicated that DOX-HK-MPEG-PCL micelles improved Dox's anti-tumor effect by enhancing tumor cell apoptosis, suppressing tumor cell proliferation, and inhibiting angiogenesis. Our data suggest that Dox-HK-MPEG-PCL micelles have the potential to be applied clinically in glioma therapy.
基金：
This work was financially supported by the National Natural Sciences Foundation of China (81502165 and
81372446) and National Major project (2013ZX09301-304-007)
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2017]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
最新[2023]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
第一作者：
第一作者机构：[1]Department of Neurosurgery, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation center, Chengdu, 610041, PR China.[2]Department of Pharmacology, Yale School of Medicine, Yale University, New Haven, Connecticut, 06520, USA.[3]Institute of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, PR China.
共同第一作者：
通讯作者：
通讯机构：[1]Department of Neurosurgery, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University and Collaborative Innovation center, Chengdu, 610041, PR China.[3]Institute of Neurosurgery, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, PR China.
推荐引用方式(GB/T 7714)：
Gao Xiang,Yu Ting,Xu Guangya,et al.Enhancing the anti-glioma therapy of doxorubicin by honokiol with biodegradable self-assembling micelles through multiple evaluations.[J].Scientific reports.2017,7:43501.doi:10.1038/srep43501.
APA：
Gao Xiang,Yu Ting,Xu Guangya,Guo Gang,Liu Xiaoxiao...&Zhou Liangxue.(2017).Enhancing the anti-glioma therapy of doxorubicin by honokiol with biodegradable self-assembling micelles through multiple evaluations..Scientific reports,7,
MLA：
Gao Xiang,et al."Enhancing the anti-glioma therapy of doxorubicin by honokiol with biodegradable self-assembling micelles through multiple evaluations.".Scientific reports 7.(2017):43501
