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The comparison genomics analysis with glioblastoma multiforme (GBM) cells under 3D and 2D cell culture conditions.

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机构： [a]State Key Lab of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou, 310058, People’s Republic of China [b]School of Mechanical Engineering, Zhejiang University, Hangzhou, 310058, People’s Republic of China [c]National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, People’s Republic of China [d]The Affiliated Stomatologic Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, People’s Republic of China [e]Department of Engineering, University of Cambridge, Trumpington Street, Cambridge, CB2 1PZ, United Kingdom [f]Zhejiang California International NanoSystems Institute, Zhejiang University, Hangzhou, 310029, People’s Republic of China

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GBM, the most common and aggressive malignant primary brain tumors which needs new research approach to reveal the underline molecular mechanism of tumor progression. The 3D in vitro tumor model can be a simple and effective way to study tumor characteristics with ability to replicate of the tumor milieu. In the current study, we adopted the DNA microarray to analyze the gene expression of GBM tumor cells cultured under 2D cell culture flasks and 3D PLA porous scaffolds for 4,7 and 14 days. For 14 day old cultures, 8117 and 3060 genes expression were upregulated and downregulated respectively. Further KEGG pathway analysis revealed, the upregulated genes were mainly enriched/involved in PPAR and PI3K-Akt signaling pathways whereas the downregulated genes were mainly contributed in metabolism, ECM related and TGF-beta pathways. Thus, our approach of establishing 3D in vitro tumor model provides realistic results and proves itself a powerful tool for understanding the inner nature of GBM and can be considered as potential platform for drug screening. Copyright © 2018 Elsevier B.V. All rights reserved.

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出版当年[2018]版：

大类 | 2 区生物

小类 | 2 区生物物理 3 区物理化学 3 区材料科学：生物材料

最新[2023]版：

大类 | 2 区

医学

小类 | 1 区生物物理 2 区物理化学 3 区材料科学：生物材料

第一作者：

第一作者机构： [a]State Key Lab of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou, 310058, People’s Republic of China [b]School of Mechanical Engineering, Zhejiang University, Hangzhou, 310058, People’s Republic of China [*1]State Key Lab of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou, 310058, People’s Republic of China.

通讯作者：

通讯机构： [a]State Key Lab of Fluid Power and Mechatronic Systems, Zhejiang University, Hangzhou, 310058, People’s Republic of China [b]School of Mechanical Engineering, Zhejiang University, Hangzhou, 310058, People’s Republic of China [*1]State Key Lab of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou, 310058, People’s Republic of China.

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