机构：[1]Key Laboratory of Bio-Resources and Eco-Environment of Ministry ofEducation, State Key Laboratory of Hydraulics and Mountain River Engineering,College of Life Sciences, Sichuan University, Chengdu 610065, China[2]NationalLaboratory of Macromolecules, Institute of Biophysics, Chinese Academy ofScience, Beijing 100101, China[3]Department of Biological Sciences, Texas TechUniversity, Lubbock, TX 79409, USA[4]Laboratory of Structural Biology and MOELaboratory of Protein Science, School of Medicine and Life Sciences, TsinghuaUniversity, Beijing 100084, China[5]Collaborative Innovation Center forBiotherapy, State Key Laboratory of Biotherapy and Cancer Center, West ChinaHospital, West China Medical School, Sichuan University, Chengdu, China四川大学华西医院
The function of abscisic acid (ABA) is mediated by its receptors termed RCARs/PYR1/PYLs. Modulation of ABA signaling is vital for plant growth and development. The RCAR-PP2C-SnRK2 regulatory modules have been defined as the core components in ABA signaling. However, it is still not clear whether and how the ABA receptors could be modified at the initial post-translational stage to fine-tune ABA transduction pathway. Here we identify and characterize the putative receptor-like cytoplasmic kinase (RLCK) in Arabidopsis named CARK1, which interacts with RCAR3 (PYL8) and RCAR11 (PYR1) in the manner of phosphorylation. Structural studies of CARK1 revealed the critical active site, N204, which accounts for the kinase activity and the direct interaction with RCAR3/RCAR11. CARK1 phosphorylates RCAR3/RCAR11 at one conserved threonine site, T77/T78. Our genetic analyses further demonstrated that CARK1 positively regulates ABA-mediated physiological responses and overexpression of CARK1 in Arabidopsis distinctly promotes the drought resistance. Moreover, the phosphor-mimic form of RCAR11 in the cark1 mutant is able to functionally complement the ABA sensitivity. CARK1 positively regulates ABA-responsive gene expression and enhances RCAR3/RCAR11's inhibition to Clade A PP2C. Taken together, our studies strongly support the functional significance of CARK1 in positively regulating ABA signaling via phosphorylation on RCAR3/RCAR11 in Arabidopsis.
基金：
This study was funded by the NSFC31671455 (Y.Y.), 973 Projects
2015CB755702 (Y.Y.), national transgenic project 2016ZX08009003-002-001 (X.
L.), 2013CB911103 (Z.L.), and NSFC81322023 (Z.L.).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|2 区生物
小类|3 区细胞生物学
最新[2023]版：
大类|1 区生物学
小类|2 区细胞生物学
第一作者：
第一作者机构：[1]Key Laboratory of Bio-Resources and Eco-Environment of Ministry ofEducation, State Key Laboratory of Hydraulics and Mountain River Engineering,College of Life Sciences, Sichuan University, Chengdu 610065, China
通讯作者：
通讯机构：[4]Laboratory of Structural Biology and MOELaboratory of Protein Science, School of Medicine and Life Sciences, TsinghuaUniversity, Beijing 100084, China[5]Collaborative Innovation Center forBiotherapy, State Key Laboratory of Biotherapy and Cancer Center, West ChinaHospital, West China Medical School, Sichuan University, Chengdu, China
推荐引用方式(GB/T 7714)：
Liang Zhang,Xiaoyi Li,Dekuan Li,et al.CARK1 mediates ABA signaling by phosphorylation of ABA receptors.[J].Cell discovery.2018,4:30.doi:10.1038/s41421-018-0029-y.
APA：
Liang Zhang,Xiaoyi Li,Dekuan Li,Yuna Sun,Ying Li...&Yi Yang.(2018).CARK1 mediates ABA signaling by phosphorylation of ABA receptors..Cell discovery,4,
MLA：
Liang Zhang,et al."CARK1 mediates ABA signaling by phosphorylation of ABA receptors.".Cell discovery 4.(2018):30
