机构：[1]State Key Laboratory of Oral Diseases, Sichuan University, 610041, Chengdu, China.[2]Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.[3]National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, 610041, China.[4]Department of Laboratory Animal Science, College of Basic Medical Sciences, Third Military Medical University, Chongqing, 400038, China.[5]Shanghai Majorbio Bio-pharm Technology Co., Ltd, Shanghai, 201203, China.[6]Department of Immuno-Oncology, Beckman Research Institute, City of Hope Comprehensive Cancer Center, Duarte, CA, 91010, USA.
Oral microbiome is potentially correlated with many diseases, such as dental caries, periodontitis, oral cancer and some systemic diseases. Twin model, as an effective method for studying human microbiota, is widely used in research of relationship between oral microbiota and dental caries. However, there were few researches focusing on caries discordant twins. In this study, in vitro assays were conducted combined with 16S rRNA sequencing analysis on oral microbiota sampled from twins who presented discordant caries experience and mice model was developed as well. Results showed that oral microbiota from caries-active twin possessed higher metabolic activity and produced more lactic production. 16S rRNA sequencing analysis showed that more than 80% of family taxa could be transferred into gnotobiotic-mice. Key caries-associated genera were significantly different between twins and the same difference in genus level could be found in mice as well (p < 0.05). This study suggested that oral microbiota of twins could be distinguished from each other despite the similarities in genetic make-up, living environment, and lifestyle. The difference in microbiota was applied to develop a mice model which may facilitate the investigation of core microbiota of dental caries.
基金：
This study was supported by the National Key Research Program of China (2017YFC0840100 and
2017YFC0840107) (L.C.); National Natural Science Foundation of China grant 81600858 (B.R.), and 81430011
(X.Z.); Recruitment Program for Young Professionals (M.F.) the Youth Grant of the Science and Technology
Department of Sichuan Province, China 2017JQ0028 (L.C.). Innovative Research Team Program of Sichuan
Province (L.C.).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
最新[2023]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
第一作者：
第一作者机构：[1]State Key Laboratory of Oral Diseases, Sichuan University, 610041, Chengdu, China.[2]Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.[3]National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, 610041, China.
共同第一作者：
通讯作者：
通讯机构：[1]State Key Laboratory of Oral Diseases, Sichuan University, 610041, Chengdu, China.[2]Department of Cariology and Endodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.[3]National Clinical Research Center for Oral Diseases, Sichuan University, Chengdu, 610041, China.
推荐引用方式(GB/T 7714)：
Wu Hongle,Zeng Benhua,Li Bolei,et al.Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study.[J].Scientific reports.2018,8(1):7267.doi:10.1038/s41598-018-25636-w.
APA：
Wu Hongle,Zeng Benhua,Li Bolei,Ren Biao,Zhao Jianhua...&Zhou Xuedong.(2018).Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study..Scientific reports,8,(1)
MLA：
Wu Hongle,et al."Research on oral microbiota of monozygotic twins with discordant caries experience - in vitro and in vivo study.".Scientific reports 8..1(2018):7267
