机构：[1]West China Hospital Emergency Department (WCHED), State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University,and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, China四川大学华西医院[2]CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology, Chinese Academy of Sciences, 100101 Beijing, China[3]Disaster Medicine Center, Sichuan University, Chengdu, 610041 Sichuan,China[4]Savaid Medical School, University of Chinese Academy of Sciences, 100049 Beijing, China[5]National Institute for Viral Disease Control andPrevention, Chinese Center for Disease Control and Prevention (China CDC), 102206 Beijing, China[6]Research Network of Immunity and Health (RNIH),Beijing Institutes of Life Science, Chinese Academy of Sciences, 100101 Beijing, China
The T3SS chaperone CesT is recently shown to interact with the post-transcriptional regulator CsrA to modulate post-attachment signaling in enteropathogenic and enterohemorrhagic Escherichia coli. The molecular basis of the CesT/CsrA binding, however, remains elusive. Here, we show that CesT and CsrA both created two ligand binding sites in their homodimers, forming irregular multimeric complexes in solution. Through construction of a recombinant CsrA-dimer (Re-CsrA) that contains a single CesT binding site, the atomic binding features between CesT and CsrA are delineated via the structure of the CesT/Re-CsrA complex. In contrast to a previously reported N-terminally swapped dimer-form, CesT adopts a dimeric architecture with a swapped C-terminal helix for CsrA engagement. In CsrA, CesT binds to a surface patch that extensively overlaps with its mRNA binding site. The binding mode therefore justifies a mechanism of CsrA-modulation by CesT via competitive inhibition of the CsrA/mRNA interactions.
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外文
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出版当年[2018]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
最新[2023]版：
大类|1 区综合性期刊
小类|1 区综合性期刊
第一作者：
第一作者机构：[1]West China Hospital Emergency Department (WCHED), State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University,and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, China
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推荐引用方式(GB/T 7714)：
Ye Fei,Yang Fanli,Yu Ruijie,et al.Molecular basis of binding between the global post-transcriptional regulator CsrA and the T3SS chaperone CesT.[J].Nature communications.2018,9(1):1196.doi:10.1038/s41467-018-03625-x.
APA：
Ye Fei,Yang Fanli,Yu Ruijie,Lin Xi,Qi Jianxun...&Lu Guangwen.(2018).Molecular basis of binding between the global post-transcriptional regulator CsrA and the T3SS chaperone CesT..Nature communications,9,(1)
MLA：
Ye Fei,et al."Molecular basis of binding between the global post-transcriptional regulator CsrA and the T3SS chaperone CesT.".Nature communications 9..1(2018):1196
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