机构：[1]National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, China.四川大学华西医院[2]Safety Evaluation Center, Sichuan Institute for Food and Drug Control, Chengdu, China.[3]Department of Thoracic Surgery, Wuxi People’s Hospital of Nanjing Medical University, Wuxi, China.[4]Laboratory of Non-Human Primate Disease Model research, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, China.四川大学华西医院[5]Department of Thoracic Surgery, West China Hospital, Sichuan University, Chengdu, China.四川大学华西医院
Iron oxide nanoparticles (IONPs) have been increasingly used in biomedical applications, but the comprehensive understanding of their interactions with biological systems is relatively limited. In this study, we systematically investigated the in vitro cell uptake, cytotoxicity, in vivo distribution, clearance and toxicity of commercially available and well-characterized IONPs with different sizes and coatings. Polyethylenimine (PEI)-coated IONPs exhibited significantly higher uptake than PEGylated ones in both macrophages and cancer cells, and caused severe cytotoxicity through multiple mechanisms such as ROS production and apoptosis. 10 nm PEGylated IONPs showed higher cellular uptake than 30 nm ones, and were slightly cytotoxic only at high concentrations. Interestingly, PEGylated IONPs but not PEI-coated IONPs were able to induce autophagy, which may play a protective role against the cytotoxicity of IONPs. Biodistribution studies demonstrated that all the IONPs tended to distribute in the liver and spleen, and the biodegradation and clearance of PEGylated IONPs in these tissues were relatively slow (>2 weeks). Among them, 10 nm PEGylated IONPs achieved the highest tumor uptake. No obvious toxicity was found for PEGylated IONPs in BALB/c mice, whereas PEI-coated IONPs exhibited dose-dependent lethal toxicity. Therefore, it is crucial to consider the size and coating properties of IONPs in their applications.
基金：
National Natural Science Foundation of China (81101143, 81572617,
and 81630101), the Basic Research Program from the Science and Technology Bureau of Sichuan Province
(2015JY0197), the Sichuan University Outstanding Young Scholars Research Grant (2014SCU04A13), and the
basic research grant from the Health and Family Planning Commission of Sichuan Province (150120).
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2018]版：
大类|3 区综合性期刊
小类|3 区综合性期刊
最新[2023]版：
大类|2 区综合性期刊
小类|2 区综合性期刊
第一作者：
第一作者机构：[1]National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
共同第一作者：
通讯作者：
通讯机构：[1]National Chengdu Center for Safety Evaluation of Drugs, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, China.[4]Laboratory of Non-Human Primate Disease Model research, State Key Laboratory of Biotherapy, Collaborative Innovation Center for Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
推荐引用方式(GB/T 7714)：
Feng Qiyi,Liu Yanping,Huang Jian,et al.Uptake, distribution, clearance, and toxicity of iron oxide nanoparticles with different sizes and coatings.[J].Scientific reports.2018,8(1):2082.doi:10.1038/s41598-018-19628-z.
APA：
Feng Qiyi,Liu Yanping,Huang Jian,Chen Ke,Huang Jinxing&Xiao Kai.(2018).Uptake, distribution, clearance, and toxicity of iron oxide nanoparticles with different sizes and coatings..Scientific reports,8,(1)
MLA：
Feng Qiyi,et al."Uptake, distribution, clearance, and toxicity of iron oxide nanoparticles with different sizes and coatings.".Scientific reports 8..1(2018):2082
