机构：[1]Department of Nuclear Medicine, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China[2]Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, No. 25, Taiping St, Luzhou, Sichuan 646000, China[3]Department of Hepatobiliary Surgery, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China[4]Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Taipa, Macau SAR 999056, China
This work evaluated the potential of 68Ga-labelledNOTA-ICG (1,4,7-triazacyclononane-1,4,7-triacetic acid indocyanine green) for liver reserve imaging.
To determine the optimal conditions for generating 68Ga-NOTA-ICG, various reaction parameters were implemented. Quality control analysis was performed using different chromatography techniques. The in vitro and in vivo stability was also measured at specific time points. The radioactivity ratio between n-octanol and water was determined to evaluate the water solubility of 68Ga-NOTA-ICG. The plasma-protein binding rate of the labelled compound was determined by the methanol method. The biodistribution and imaging findings were evaluated in normal animals at different time points after injection. A preliminary imaging evaluation was performed using an animal model of hepatic ischaemia-reperfusion injury, which was confirmed by pathology.
68Ga-NOTA-ICG was prepared with very high radiochemical purity (>98%) by reacting at 90°C for 10 min at pH = 3.5∼4.0, with excellent stability in vivo and in vitro (>95% 3 h postpreparation). The in vitro plasma-protein binding rate of 68Ga-NOTA-ICG was 13.01 ± 0.7%, and it showed strong water solubility (log P=-2.01 ± 0.04). We found that in addition to excretion through the biliary tract and intestines, 68Ga-NOTA-ICG can be excreted through the urinary tract. The image quality of 68Ga-NOTA-ICG was very high; imaging agent retained in the area of liver injury could clearly be observed.
This is the first report on a 68Ga-labelled NOTA-ICG fragment for liver reserve function studies. This complex has promise as a candidate agent for liver reserve imaging.
基金：
THis study was funded by the National Natural
Science Foundation (81701739) and the Luzhou Municipal
Government Foundation (2016LZXNYD-J01) of China.
语种：
外文
PubmedID：
中科院(CAS)分区：
出版当年[2019]版：
大类|4 区医学
小类|4 区核医学
最新[2023]版：
无
第一作者：
第一作者机构：[1]Department of Nuclear Medicine, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China[2]Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, No. 25, Taiping St, Luzhou, Sichuan 646000, China
共同第一作者：
通讯作者：
通讯机构：[1]Department of Nuclear Medicine, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China[2]Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, No. 25, Taiping St, Luzhou, Sichuan 646000, China
推荐引用方式(GB/T 7714)：
Yuxiao Xia,Li Zhang,Yanhong Zhao,et al.Gallium-68-Labelled Indocyanine Green as a Potential Liver Reserve Imaging Agent.[J].Contrast media & molecular imaging.2019,2019:4201353.doi:10.1155/2019/4201353.
APA：
Yuxiao Xia,Li Zhang,Yanhong Zhao,Xiangdong Liu,Liang Cai...&Wei Zhang.(2019).Gallium-68-Labelled Indocyanine Green as a Potential Liver Reserve Imaging Agent..Contrast media & molecular imaging,2019,
MLA：
Yuxiao Xia,et al."Gallium-68-Labelled Indocyanine Green as a Potential Liver Reserve Imaging Agent.".Contrast media & molecular imaging 2019.(2019):4201353
