机构：[1]Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics,West Virginia University, PO Box 9196, 64 Medical Center Drive, Morgantown,WV 26506‑9196, USA[2]Department of Orthopaedics, Zhongshan Hospitalof Fudan University, 180 Fenglin Road, Shanghai 200032, China[3]Departmentof Orthopaedics, Chengdu Military General Hospital, Chengdu 610083,Sichuan, China[4]WVU Cancer Institute, Robert C. Byrd Health Sciences Center,West Virginia University, Morgantown, WV 26506, USA.
Cellular senescence is a major hurdle for primary cell-based tissue engineering and regenerative medicine. Telomere erosion, oxidative stress, the expression of oncogenes and the loss of tumor suppressor genes all may account for the cellular senescence process with the involvement of various signaling pathways. To establish immortalized cell lines for research and clinical use, strategies have been applied including internal genomic or external matrix microenvironment modification. Considering the potential risks of malignant transformation and tumorigenesis of genetic manipulation, environmental modification methods, especially the decellularized cell-deposited extracellular matrix (dECM)-based preconditioning strategy, appear to be promising for tissue engineering-aimed cell immortalization. Due to few review articles focusing on this topic, this review provides a summary of cell senescence and immortalization and discusses advantages and limitations of tissue engineering and regeneration with the use of immortalized cells as well as a potential rejuvenation strategy through combination with the dECM approach.
第一作者机构：[1]Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics,West Virginia University, PO Box 9196, 64 Medical Center Drive, Morgantown,WV 26506‑9196, USA[2]Department of Orthopaedics, Zhongshan Hospitalof Fudan University, 180 Fenglin Road, Shanghai 200032, China
通讯作者：
通讯机构：[1]Stem Cell and Tissue Engineering Laboratory, Department of Orthopaedics,West Virginia University, PO Box 9196, 64 Medical Center Drive, Morgantown,WV 26506‑9196, USA[4]WVU Cancer Institute, Robert C. Byrd Health Sciences Center,West Virginia University, Morgantown, WV 26506, USA.
推荐引用方式(GB/T 7714)：
Wang Yiming,Chen Song,Yan Zuoqin,et al.A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration.[J].Cell & bioscience.2019,9:7.doi:10.1186/s13578-018-0264-9.
APA：
Wang Yiming,Chen Song,Yan Zuoqin&Pei Ming.(2019).A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration..Cell & bioscience,9,
MLA：
Wang Yiming,et al."A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration.".Cell & bioscience 9.(2019):7
