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Structural and Functional Basis of SARS-CoV-2 Entry by Using Human ACE2.

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机构： [1]CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China [2]Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People’s Hospital, Shenzhen 518112, China [3]CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China [4]University of the Chinese Academy of Sciences, Beijing 100049, China [5]Laboratory of Protein Engineering and Vaccines, Tianjin Institute of Biotechnology, Tianjin 300308, China [6]College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China [7]Institute of Physical Science and Information, Anhui University, Hefei 230039, China [8]West China Hospital Emergency Department (WCHED), State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, China [9]School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China [10]State Key Laboratory for Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region 999077, China [11]Department of Microbiology, The University of Hong Kong, Pokfulam, Hong Kong Special Administrative Region 999077, China [12]Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, China [13]College of Life Science, University of the Chinese Academy of Sciences, Beijing 100049, China [14]Savaid Medical School, University of the Chinese Academy of Sciences, Beijing 100049, China

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The recent emergence of a novel coronavirus (SARS-CoV-2) in China has caused significant public health concerns. Recently, ACE2 was reported as an entry receptor for SARS-CoV-2. In this study, we present the crystal structure of the C-terminal domain of SARS-CoV-2 (SARS-CoV-2-CTD) spike (S) protein in complex with human ACE2 (hACE2), which reveals a hACE2-binding mode similar overall to that observed for SARS-CoV. However, atomic details at the binding interface demonstrate that key residue substitutions in SARS-CoV-2-CTD slightly strengthen the interaction and lead to higher affinity for receptor binding than SARS-RBD. Additionally, a panel of murine monoclonal antibodies (mAbs) and polyclonal antibodies (pAbs) against SARS-CoV-S1/receptor-binding domain (RBD) were unable to interact with the SARS-CoV-2 S protein, indicating notable differences in antigenicity between SARS-CoV and SARS-CoV-2. These findings shed light on the viral pathogenesis and provide important structural information regarding development of therapeutic countermeasures against the emerging virus. Copyright © 2020 Elsevier Inc. All rights reserved.

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出版当年[2020]版：

大类 | 1 区

生物学

小类 | 1 区生化与分子生物学 1 区细胞生物学

最新[2023]版：

大类 | 1 区

生物学

小类 | 1 区生化与分子生物学 1 区细胞生物学

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出版当年[2020]版：

Q1 CELL BIOLOGY Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

最新[2023]版：

Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Q1 CELL BIOLOGY

影响因子： 45.5 最新[2023版] 49 最新五年平均 41.584 出版当年[2020版] 46.899 出版当年五年平均 38.637 出版前一年[2019版] 66.85 出版后一年[2021版]

第一作者：

第一作者机构： [1]CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China [2]Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People’s Hospital, Shenzhen 518112, China [3]CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

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通讯作者：

通讯机构： [1]CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China [2]Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Third People’s Hospital, Shenzhen 518112, China [3]CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China [7]Institute of Physical Science and Information, Anhui University, Hefei 230039, China [13]College of Life Science, University of the Chinese Academy of Sciences, Beijing 100049, China [14]Savaid Medical School, University of the Chinese Academy of Sciences, Beijing 100049, China

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Structural and Functional Basis of SARS-CoV-2 Entry by Using Human ACE2. $(function () { $("[data-toggle='tooltip']").tooltip(); });

文献详情

Structural and Functional Basis of SARS-CoV-2 Entry by Using Human ACE2.