E. coli HX88108 was isolated from a patient and found to produce plasmid-encode β-lactamases with conferring highly resistance to ceforperazone (CPZ). The β-lactamases of the E. coli HX88108 and transformants pFC, pFT 1, pFT 2 and pFT 3 were studied. The β-lactamases stability test among 11 β-lactam antibiotics showed that β-lactamases from E. coli HX88108. pFC, pFT 1, readily hydrolyzed penicillins, the first, second-generation cephalosporins and CPZ. β-lactamases of pFT 2 and pFT 3 hydrolyzed penicillins more strongly than cephalosporins. On the other hand, experiments of inhibiting enzyme were carried out. The results indicated that β-lactamases of HX88108, pTF 1, pFT 2 and pFT 3, were inhibited by clavulanic acid(CA) and sulbactam (SBT). Enzyme of pFC was inhibited poorly by CA and SBT. Through isoelectric focusing technique, the PIs were as follows: HX88108 contained three β-lactamases, of which the PIs were 5. 25,5,3 and 5. 6 respectively; the PIs of β-lactamases from pFT 2, pFT 3, were 5. 3 and 5. 6. pFC and pFT 1 were different plasmids encoded β-lactamases with the same PI 5. 25. The results indicate that the β-lactamases of E. coli HX88108 may be a new member in TEM farmily.