机构：[a]Department of Lung Tumor, Sichuan Cancer Hospital, Chengdu, Sichuan Province, China四川省肿瘤医院[b]Department of Orthopedics, West China Hospital, Chengdu, Sichuan Province, China四川大学华西医院
INTRODUCTION: Gene expression profiles of peripheral blood monocytes from patients with non-small cell lung cancer (NSCLC) before and after chemotherapy were used to investigate the effect of chemotherapy on gene expression. MATERIALS AND METHODS: Microarray dataset GSE39345 was downloaded from Gene Expression Omnibus, including 32 NSCLC samples before chemotherapy, 17 NSCLC samples after chemotherapy and 20 healthy samples. Raw data pretreatment and differentially expressed genes (DEGs) analysis between health and NSCLC samples before chemotherapy, health and NSCLC samples after chemotherapy were performed with packages of R. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was done for the common DEGs with WebGestalt to investigate their underlying function. RESULTS: A total of 904 DEGs were identified in health vs. NSCLC samples before chemotherapy and 2148 in health vs. NSCLC samples after chemotherapy. Further, they were divided into three sets: 584 common DEGs, 320 unique DEGs (health vs NSCLC samples before chemotherapy), and 1564 unique DEGs (health vs NSCLC samples after chemotherapy). Function enrichment analysis showed that these common DEGs were associated with cell cycle and cell differentiation. CONCLUSIONS: Chemotherapy could not completely reverse the lung cancer development because several cell growth-related genes are still present even after chemotherapy.
基金：
Health Department of Sichuan
Provincial Research Project (No.120038).
语种：
外文
中科院(CAS)分区：
出版当年[2014]版：
大类|4 区医学
小类|4 区药学
最新[2023]版：
大类|4 区医学
小类|4 区药学
第一作者：
第一作者机构：[a]Department of Lung Tumor, Sichuan Cancer Hospital, Chengdu, Sichuan Province, China
推荐引用方式(GB/T 7714)：
Li J,Shi R,Yu P.Effects of chemotherapy on global gene expression in non-small cell lung cancer[J].European Review for Medical and Pharmacological Sciences.2014,18(1):
APA：
Li, J,Shi, R&Yu, P.(2014).Effects of chemotherapy on global gene expression in non-small cell lung cancer.European Review for Medical and Pharmacological Sciences,18,(1)
MLA：
Li, J,et al."Effects of chemotherapy on global gene expression in non-small cell lung cancer".European Review for Medical and Pharmacological Sciences 18..1(2014)
