机构：[1]State Key Laboratory for Supramolecular Structure and Materials, Jilin University.[2]The State Engineering Laboratory of AIDS Vaccine, Jilin University.[3]Sichuan Tumor Hospital & Institute.四川省肿瘤医院[4]State Key Laboratory for Supramolecular Structure and Materials, Jilin UniVersity, No. 2699, Qianjin Street, Changchun 130012 China, The State Engineering Laboratory of AIDS Vaccine, Jilin UniVersity, No. 2699, Qianjin Street, Changchun 130012 China, and Sichuan Tumor Hospital & Institute, Chengdu 610041 China四川省肿瘤医院
Survivin, as an apoptosis suppressor, exists as a homodimer interfacing at the N-terminal portion (residues 6-13) of its baculovirus IAP repeat (BIR) domain and a linker segment (residues 89-102). Here we expressed full-length human Survivin (SurF) and a series of its mutants, Sur Delta N7, Sur Delta N13, and Sur Delta N18 with significant truncations of the N-terminus, all of which could still dimerize in solution. Single-molecule force spectroscopy (SMFS) was used to quantitate the unbinding forces of full-length and the mutant homodimers and revealed that the N-terminal residues up to Arg18 were not essential for dimerization. Meanwhile, the binding of Sur Delta N7 to Smac/DIABLO determined by ELISA was as efficient as the wild-type, but that of Sur Delta N13 was significantly reduced, and that of Sur Delta N18 was completely lost. Together, these findings provide direct evidence that the N-terminal sequence of Survivin is not critical for dimer formation but may contribute to correct folding and function of BIR.
基金：
Natural Science Foundation of China (Nos.
20934002 and 20973073), Jilin Province Natural Science
Foundation (20070926-01), the National Basic Research Program
(2007CB808006), the Programs for New Century Excellent
Talents in University (NCET), and the 111 Project
(B06009).
第一作者机构：[1]State Key Laboratory for Supramolecular Structure and Materials, Jilin University.[4]State Key Laboratory for Supramolecular Structure and Materials, Jilin UniVersity, No. 2699, Qianjin Street, Changchun 130012 China, The State Engineering Laboratory of AIDS Vaccine, Jilin UniVersity, No. 2699, Qianjin Street, Changchun 130012 China, and Sichuan Tumor Hospital & Institute, Chengdu 610041 China
通讯作者：
通讯机构：[1]State Key Laboratory for Supramolecular Structure and Materials, Jilin University.[4]State Key Laboratory for Supramolecular Structure and Materials, Jilin UniVersity, No. 2699, Qianjin Street, Changchun 130012 China, The State Engineering Laboratory of AIDS Vaccine, Jilin UniVersity, No. 2699, Qianjin Street, Changchun 130012 China, and Sichuan Tumor Hospital & Institute, Chengdu 610041 China
推荐引用方式(GB/T 7714)：
Gao Yang,Zhang Huafei,Zhang Min,et al.N-Terminal Deletion Effects of Human Survivin on Dimerization and Binding to Smac/DIABLO in Vitro[J].JOURNAL OF PHYSICAL CHEMISTRY B.2010,114(47):15656-15662.doi:10.1021/jp1036603.
APA：
Gao, Yang,Zhang, Huafei,Zhang, Min,Zhang, Haihong,Yu, Xianghui...&Wu, Yuqing.(2010).N-Terminal Deletion Effects of Human Survivin on Dimerization and Binding to Smac/DIABLO in Vitro.JOURNAL OF PHYSICAL CHEMISTRY B,114,(47)
MLA：
Gao, Yang,et al."N-Terminal Deletion Effects of Human Survivin on Dimerization and Binding to Smac/DIABLO in Vitro".JOURNAL OF PHYSICAL CHEMISTRY B 114..47(2010):15656-15662
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