机构：[1]Engineering Research Center of Biopolymer Functional Materials of Yunnan, Yunnan University of Nationalities, Kunming, Yunnan, China[2]Department of Radiation Oncology, Sichuan Cancer Hospital, Chengdu, Sichuan, China四川省肿瘤医院
Cathelicidin-BF-30 (BF-30), a water-soluble peptide isolated from the snake venom of Bungarus fasciatus containing 30 amino acid residues, was incorporated in poly(D,L-lactide-co-glycolide) (PLGA) 75:25 microspheres (MS) prepared by a water in oil in water W/O/W emulsification solvent extraction method. The aim of this work was to investigate the stability of BF-30 after encapsulation. D-trehalose was used as an excipient to stabilize the peptide. The MS obtained were mostly under 2 mu m in size and the encapsulation efficiency was 88.50 +/- 1.29%. The secondary structure of the peptide released in vitro was determined to be nearly the same as the native peptide using Circular Dichroism (CD). The ability of BF-30 to inhibit the growth of Escherichia coli was also maintained. The cellular relative growth and hemolysis rates were 92.16 +/- 3.55% and 3.52 +/- 0.45% respectively.
基金：
National Natural Science Foundation of China [31160198, 31360417]; Applied Basic Research Key Project of Yunnan [2013FA039]; Applied Basic Research Project of Yunnan [2011FB084]; High-End Technology Professionals Introduction Plan in Yunnan province [2010CI119]
第一作者机构：[1]Engineering Research Center of Biopolymer Functional Materials of Yunnan, Yunnan University of Nationalities, Kunming, Yunnan, China
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推荐引用方式(GB/T 7714)：
Li Lili,Wang Qifeng,Li Hongli,et al.Preparation, Characterization, In Vitro Release and Degradation of Cathelicidin-BF-30-PLGA Microspheres[J].PLOS ONE.2014,9(6):doi:10.1371/journal.pone.0100809.
APA：
Li, Lili,Wang, Qifeng,Li, Hongli,Yuan, Mingwei&Yuan, Minglong.(2014).Preparation, Characterization, In Vitro Release and Degradation of Cathelicidin-BF-30-PLGA Microspheres.PLOS ONE,9,(6)
MLA：
Li, Lili,et al."Preparation, Characterization, In Vitro Release and Degradation of Cathelicidin-BF-30-PLGA Microspheres".PLOS ONE 9..6(2014)
